Figure 7个  Table 1个
    • Protein ID Protein name Description Unique peptidesa Sequence coverage (%)b Abundancec Mass(kDa) Subcellular locationd Reported in other virusese
      O92284 G Glycoprotein 37 49 1.75E+10 58.86 Virion
      Q8JXF6 N Nucleoprotein 40 80.7 1.48E+10 50.73 Virion
      P22363 P Phosphoprotein 32 84.2 1.13E+10 33.62 Virion
      P25223 M Matrix protein 8 52.5 7.75E+09 23.13 Virion
      D8VEC2 L Large structural protein 111 61.5 3.94E+09 242 Virion
      P40240 CD9 CD9 antigen 7 26.5 1.72E+09 25.26 Membrane IAV1, HAV, MEV, HIV1, ASFV
      P63168 DLC8 Dynein light chain 1 2 57.3 1.09E+09 10.37 Nucleus, mitochondria, cytoskeleton
      P63017 HSC70 Heat shock cognate 71 kDa protein 28 55.9 5.83E+08 70.87 Membrane, nucleus, cytoplasm RSV, HIV1, HIV2, VSV, RVFV, HSV, ASFV, JUNV
      P17742 CyPA Cyclophilin A 9 68.3 5.65E+08 17.97 Cytoplasm IAV1, MEV, HIV1, HIV2, HIV3, HSV, KSHV
      P63001 RAC1 RAS-related C3 botulinum 6 36.5 3.51E+08 23.43 Membrane, cytoplasm RVFV, HIV2
      P18760 Cofilin-1 Cofilin-1 14 58.1 3.27E+08 24.58 Cytoskeleton IAV1, RSV, HIV1, HIV2, HSV
      P35762 CD81 CD81 antigen C 4 30.9 2.99E+08 25.81 Membrane IAV1, HCV, MEV, HIV1, HIV2, VV
      P61205 ARF3 ADP-ribosylation factor 3 4 57.5 2.93E+08 20.60 Golgi HSV
      P0CG50 Ubc Polyubiquitin-C 7 73.6 2.41E+08 82.55 Nucleus, cytoplasm IAV2, RSV, HIV1, VSV, JUNV
      O08992 Syntenin-1 Syntenin-1 8 40.5 1.77E+08 32.38 Membrane, cytoplasm, cytoskeleton, nucleus, ER, junction HIV2
      Q9WVE8 PACSIN 2 Protein kinase C and casein kinase substrate in neurons protein 2 13 27 1.45E+08 55.83 Cytosol, endosome, nucleus, caveola
      P10852 Slc3a2 4F2 cell-surface antigen heavy chain 19 39.7 1.03E+08 58.34 Membrane RSV, HIV1, VSV
      O35566 CD151 CD151 antigen 5 17 8.78E+07 28.25 Membrane RVFV
      P41731 CD63 CD63 antigen 3 10 8.72E+07 26.78 Membrane HCV
      Q9R0P5 Destrin Destrin 6 41.2 8.62E+07 18.52 Cytoskeleton IAV1
      P60766 CDC42 Cell division control protein 42 homolog 6 42.4 8.24E+07 21.26 Membrane, cytoskeleton, cytoplasm IAV2, HIV1, SARS
      Q9D8B3 CHMP4B Charged multivesicular body protein 4b 7 41.1 6.07E+07 24.94 Late endosome, cytosol HAV
      P63037 HSP40 DnaJ homolog subfamily A member 1 11 36.5 5.91E+07 44.87 Membrane, nucleus, ER, mitochondria, cytoplasm HIV2
      P16858 GAPDH Glyceraldehyde-3-phosphate dehydrogenase 9 30.6 5.73E+07 38.65 Cytoskeleton, cytosol, nucleus IAV1, RSV, HIV1, HIV2, HIV3, RVFV, IBV, ASFV, KSHV
      P63242 EIF5a Eukaryotic translation initiation factor 5A-1 6 45.5 5.71E+07 16.83 Nucleus, ER KSHV
      Q4VAE6 RhoA Ras family member A 5 28.5 5.67E+07 21.80 Nucleus, ER, cytoplasm IBV
      O54946 HSJ-2 DnaJ homolog subfamily B member 6 6 21.9 5.16E+07 39.81 Nucleus
      P63024 VAMP3 Vesicle-associated membrane protein 3 2 32 4.84E+07 11.48 Membrane
      Q9WU78 ALIX Programmed cell death 6-interacting protein 30 38.9 4.84E+07 96.31 Cytoskeleton, cytosol HAV, HIV1, VSV, HSV, JUNV
      P63101 14-3-3 ζ/θ 14-3-3 protein zeta/delta 6 37.6 4.66E+07 27.77 Cytoskeleton HIV1, HSV, KSHV, SARS
      Q62167 DDX3X ATP-dependent RNA helicase DDX3X 5 41.2 4.13E+07 73.10 Mitochondria, nucleus HSV, JUNV, SARS
      P99024 Tubulin β-5 Tubulin beta-5 chain 4 35.8 4.05E+07 49.67 Cytoplasm, cytoskeleton, microtubules IAV1, RSV, HIV1, VSV, ASFV
      P11499 HSP90β Heat shock protein HSP 90-beta 15 34.4 3.94E+07 83.28 Membrane, cytoplasm, nucleus RSV, HAV, HIV1, VSV, RVFV, IBV, KSHV, SARS
      Q91ZR2 SNX Sorting nexin-18 10 20 3.76E+07 67.79 Membrane
      Q61187 TSG101 Tumor susceptibility gene 101 protein 7 19.2 3.40E+07 44.12 Endosome, nucleus HIV1, VSV, JUNV
      P17182 ENO1 Alpha-enolase 9 24 3.17E+07 47.14 Membrane, cytoplasm IAV1, HAV, MEV, HIV1, HIV2, VSV, IBV, ASFV, KSHV
      Q9DB34 CHMP2A Charged multivesicular body protein 2a 5 18 3.09E+07 25.13 Cytoplasm, late endosomes
      Q9Z127 SLC7 Large neutral amino acids transporter small subunit 1 5 8.8 2.94E+07 55.87 Membrane, cytosol
      P35278 Rab5C Ras-related protein Rab-5C 4 19.7 2.91E+07 25.35 Membrane, endosomes HAV, HIV1, HIV2, ASFV, HSV
      Q3UFR4 SLC1 Amino acid transporter 8 18.9 2.60E+07 58.36 Membrane
      Q99J93 IFITM Interferon-induced transmembrane protein 2 2 23.6 2.30E+07 15.74 Membrane
      Q9D1C8 VPS28 Vacuolar protein sorting-associated protein 28 homolog 7 36.7 2.19E+07 25.45 Endosomes HIV2
      P51150 RAB7A Ras-related protein Rab-7a 6 32.9 1.88E+07 23.49 Late endosomes HAV, HIV1, RVFV, HSV, KSHV, JUNV
      P06837 GAP43 Neuromodulin 7 49.8 1.79E+07 23.63 Membrane, synapses
      P26040 Ezrin Ezrin 8 18.3 1.77E+07 69.41 Cytoskeleton, cytosol IAV2, HIV1
      P60335 PCBP1 Poly(rC)-binding protein 1 4 18.5 1.64E+07 37.5 Nucleus, cytoplasm HIV1, HIV2, SARS
      P16045 Galectin-1 Galectin-1 3 28.1 1.61E+07 14.87 Cell surface, extracellular matrix HIV2
      P62331 ARF6 ADP-ribosylation factor 6 3 21.1 1.11E+07 20.08 Cytoplasm, cytosol, early endosomes
      Q91YD9 nWASP Neural Wiskott-Aldrich syndrome protein 5 12.6 9.01E+06 54.27 Cytoplasm, cytoskeleton, nucleus
      P61089 Ube2 Ubiquitin-conjugating enzyme E2 N 5 33.6 6.31E+06 17.14 Cytoplasm, nucleus
      B2RRX1 β-actin Beta-actin 3 54.1 4.96E+06 41.74 Membrane, cytoskeleton, cytosol IAV1, HIV1, HIV2, VSV, RVFV, IBV, HSV, KSHV
      Q8R0J7 VPS37B Vacuolar protein sorting-associated protein 37B 4 17.9 4.30E+06 31.06 Late endosomes, cytoplasm
      P46467 VPS4B Vacuolar protein sorting-associated protein 4B 7 18.2 3.62E+06 49.42 Late endosomes HAV
      P68040 RACK1 Receptor of activated protein C kinase 1 4 11.7 2.58E+06 35.08 Membrane, cytoplasm, nucleus
      aThe number of unique peptides correspond to the maximal values among the three biological replications.
      bThe percentages of sequence coverage based on peptides with unique sequences.
      cAverage abundance expressed by iBAQ calculated from three separate determinations.
      dSubcellular location was investigated using the Uniprot database. Endoplasmic reticulum, ER.
      eVirus names: influenza A virus (Shaw et al.2008 for IAV1, Mindaye et al. 2017 for IAV2); HAV: hepatitis A virus (McKnight et al.2017); MEV: measles virus (Sviben et al.2018); HIV: human immunodeficiency virus (Linde et al.2013 for HIV1, Chertova et al. 2006 for HIV2, Saphire et al. 2006 for HIV3); ASFV: African swine fever virus (Alejo et al.2018); RSV: respiratory syncytial virus (Radhakrishnan et al.2010)); VSV: vesicular stomatitis virus (Moerdyk-Schauwecker et al.2009); RVFV: rift Valley fever virus (Nuss et al.2014); HSV: herpes simplex virus type 1 (Stegen et al.2013); KSHV: Kaposi's sarcoma-associated herpesvirus (Zhu et al.2005); HCV: hepatitis C virus (Lussignol et al.2016); VV: vaccinia virus (Krauss et al.2002); IBV: infection bronchitis virus (Kong et al.2010); JUNV: Junin virus (Ziegler et al.2018); SARS: severe acute respiratory syndrome (Neuman et al.2008).

      Table 1.  High-confidence proteins identified in purified RABV virions by LC–MS/MS.