Figure 4个  Table 4个
    • Period of sample collection Sampling site (county) Mosquito species Total Midges
      Culex tritaeniorhynchus Culex quinquefasciatus Armigeres subalbatus Anopheles sinensis
      June Xiajiang 3130
      (1530/1600)a      		 0 80 830
      (730/100)      		 3960
      (2260/1700)      		 3000
      Yongfeng 5100
      (2100b/3000)      		 0 0 2600
      (1250b/1350)      		 7700
      (3350/4350)      		 1100
      Anfu 530b 0 0 500b 1030 550
      Jing 55 39 0 33 127 4550
      Gangshan (1900/2650)
      Subtotal 8815
      (4215/4600)      		 39 80 3963
      (2513/1450)      		 12897
      (6847/6050)      		 9200
      (6550/2650)
      July Lichuan 270 0 0 230 500 12400
      Zixi 15 0 6 91 112 7800
      Long-Hushan town 520 0 44 197b 761 8900
      Yanshan 5150 0 370 1100 6620 10600
      Yushan 1660 0 12 200 1872 5300
      Fuliang 162 0 20 41 223 3300
      Subtotal 7777 0 452 1859 10088 48300
      Total 16592
      (11992/4600)      		 39 532 5822
      (4372/1450)      		 22985
      (16935/6050)      		 57500
      (54850/2650)
      a(1530/1600): (number of ground mosquito specimens/number of unground mosquito specimens).
      bZIKV isolated from this mosquito specimen.

      Table 1.  Specimens of blood-sucking insects collected in Jiangxi Province in 2018.

    • Sampling date Sampling site (county) Strain Mosquito species Breeding ground GenBank Accession No.
      June 2018.06.12 Yongfeng JXJA1835-1 Anopheles sinensis Cowshed MK696546
      2018.06.12 Yongfeng JXJA1835-2 Anopheles sinensis Cowshed MK696547
      2018.06.12 Yongfeng JXJA1839-1 Culex tritaeniorhynchus Cowshed MK696548
      2018.06.13 Anfu JXJA1866 Anopheles sinensis Sheepfold MK696549
      2018.06.13 Anfu JXJA1874-1 Culex tritaeniorhynchus Cowshed MK696550
      July 2018.07.10 Long-Hushan town JXLHS1807 Anopheles sinensis Pigsty MK696551

      Table 2.  ZIKV strains isolated from mosquitoes in Jiangxi Province in 2018.

    • Collection date Collection site (county) Mosquito species No. Individuals/no.pools No. Positive Pools/no.pools MIR(/1000)
      June Yongfeng Culex tritaeniorhynchus 2100/14 1/14 0.48
      Yongfeng Anopheles sinensis 1250/8 2/8 1.60
      Anfu Culex tritaeniorhynchus 530/5 1/5 1.89
      Anfu Anopheles sinensis 500/5 1/5 2.00
      July Long-Hushan town Anopheles sinensis 197/4 1/4 5.08
      Subtotal Culex tritaeniorhynchus 2630/19 2/19 0.76
      Anopheles sinensis 1947/17 4/17 2.05

      Table 3.  ZIKV infection rate of Cx. tritaeniorhynchus and An. sinensis.

    • Genotype Strain Source Homology of nucleotide (amino acid) of virus Divergences of amino acids
      Country Year Host JXLHS1807(ORF) JXLHS 1807(E) D683E V763M T777M S139N M/t2634V
      Africa Ⅰ MR766 Uganda 1947 Rhesus money 88.4% (96.2%) D V T s M
      Africa Ⅱ ArD7117 Senegal 1968 Aedes luteocephalus 88.4% (96.3%) D V T s M
      Asia Ⅰ P6-740 Malaysia 1966 Aedes aegypti 95.3% (98.4%) D V T s T
      Asia Ⅰ GZDJ1666-2 China 2016 Armigeres subalbatus 99.9% (99.7%) E M M N M
      Asia Ⅱ GZDJ1685 China 2016 Culex quinquefasciatus 99.9% (99.7%) E M M N M
      Asia Ⅱ SZ-WIV01 Americ an-S amon 2016 Human 99.7% (99.5%) E M M N M
      Asia Ⅱ ECMN2007 Micronesia 2007 Human 97.8% (98.8%) E V T S M
      Asia Ⅱ H/PF/2013 French-Polynesia 2013 Human 99.5% (99.4%) E M M N M
      Asia Ⅱ 1_0035_PF French-Polynesia 2014 Human 99.4% (99.4%) E M M N M
      Asia Ⅱ ZikaSPH2015 Brazil 2015 Human 99.3% (99.2%) E M M N V
      Asia Ⅱ JXLHS1807a China 2018 Anopheles sinensis E M M N M
      Asia Ⅱ JXJA1835-1a China 2018 Anopheles sinensis 99.7% (99.2%) E M M
      Asia Ⅱ JXJA1835-2a China 2018 Anopheles sinensis 99.8% (99.4%) E M M
      Asia Ⅱ JXJA1839-1a China 2018 Culex tritaeniorhynchus 99.8% (99.4%) E M M
      Asia Ⅱ JXJA1866a China 2018 Anopheles sinensis 99.8% (99.4%) E M M
      Asia Ⅱ JXJA1874-1a China 2018 Culex tritaeniorhynchus 99.8% (99.4%) E M M
      aZIKV strains isolated in this study.

      Table 4.  Variation in the amino acid sequences of ZIKVs isolated from Cx. tritaeniorhynchus and An. sinensis.