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Herpesvirus 2-D Multiplex qPCR assay (%) Single qPCR assay (%) Concordance rate (%) Positive EBV 19/149 (12.8) 19/149 (12.8) 100 HCMV 6/149 (4.0) 6/149 (4.0) 100 HHV-6A/B 0/149(0) 0/149 (0) 100 HHV-7 1/149 (0.7) 1/149 (0.7) 100 KSHV 4/149 (2.7) 4/149 (2.7) 100 HSV-2 & CMV 5/149 (3.4) 5/149 (3.4) 100 HSV-2 & EBV 8/149 (5.4) 8/149 (5.4) 100 EBV & HCMV 8/149 (5.4) 8/149 (5.4) 100 HHV-7 & KSHV 1/149 (0.7) 1/149 (0.7) 100 Negative 97/149 (65.1) 97/149 (65.1) 100 Total 149 /149 (100) 149 /149 (100) 100 Table 1. Evaluation of the single-tube nonuple qPCR assay for nine human herpesviruses and the reference gene GAPDH using 149 whole-blood samples.
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Herpes virus 2-D Multiplex qPCR assay (%) Single qPCR assay (%) Concordance rate (%) Positive HSV-1 4/21 (19.0) 4/21 (19.0) 100 HSV-2 3/21 (14.3) 3/21 (14.3) 100 VZV 7/21 (33.3) 7/21 (33.3) 100 HSV-2 & EBV 1/21 (4.8) 1/21 (4.8) 100 HSV-2 & HCMV 1/21 (4.8) 1/21 (4.8) 100 HSV-2 & HHV-7 1/21(4.8) 1/21(4.8) 100 VZV & EBV 1/21 (4.8) 1/21 (4.8) 100 VZV & KSHV 1/21 (4.8) 1/21 (4.8) 100 HSV-1 & HSV-2 & HCMV 1/21 (4.8) 1/21 (4.8) 100 HSV-2 & VZV & HHV-7 & KSHV 1/21 (4.8) 1/21 (4.8) 100 Negative 0/21(0) 0/21 (0) 100 Total 21/21 (100) 21/21 (100) 100 Table 2. Evaluation of the single-tube nonuple qPCR assay for nine human herpesviruses and the reference gene GAPDH using 21 vesicular fluid samples.
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Proportion in all samples (%) Proportion of positive samples (%) HHV single infection rate 25.9 60.3 HHVs double infection rate 15.9 37 HHV multiple infections rate 1.2 2.7 Common HHV single infection rate EBV 11.2 26 VZV 4.1 9.6 CMV 3.5 8.2 KSHV 2.3 5.5 HSV-1 2.3 5.5 HSV-2 1.7 4.1 High proportion of HHV among multiple infections HSV-2 10.6 24.7 EBV 10.6 24.7 CMV 8.8 20.5 VZV 1.8 4.1 HHV-7 1.8 4.1 KSHV 1.8 4.1 HSV-1 0.6 1.4 A total of 170 samples were tested Table 3. Detection rates of various HHVs.
Figure 3 个
Table 3 个