Figure 3个  Table 3
    • Herpesvirus 2-D Multiplex qPCR assay (%) Single qPCR assay (%) Concordance rate (%)
      Positive EBV 19/149 (12.8) 19/149 (12.8) 100
      HCMV 6/149 (4.0) 6/149 (4.0) 100
      HHV-6A/B 0/149(0) 0/149 (0) 100
      HHV-7 1/149 (0.7) 1/149 (0.7) 100
      KSHV 4/149 (2.7) 4/149 (2.7) 100
      HSV-2 & CMV 5/149 (3.4) 5/149 (3.4) 100
      HSV-2 & EBV 8/149 (5.4) 8/149 (5.4) 100
      EBV & HCMV 8/149 (5.4) 8/149 (5.4) 100
      HHV-7 & KSHV 1/149 (0.7) 1/149 (0.7) 100
      Negative 97/149 (65.1) 97/149 (65.1) 100
      Total 149 /149 (100) 149 /149 (100) 100

      Table 1.  Evaluation of the single-tube nonuple qPCR assay for nine human herpesviruses and the reference gene GAPDH using 149 whole-blood samples.

    • Herpes virus 2-D Multiplex qPCR assay (%) Single qPCR assay (%) Concordance rate (%)
      Positive HSV-1 4/21 (19.0) 4/21 (19.0) 100
      HSV-2 3/21 (14.3) 3/21 (14.3) 100
      VZV 7/21 (33.3) 7/21 (33.3) 100
      HSV-2 & EBV 1/21 (4.8) 1/21 (4.8) 100
      HSV-2 & HCMV 1/21 (4.8) 1/21 (4.8) 100
      HSV-2 & HHV-7 1/21(4.8) 1/21(4.8) 100
      VZV & EBV 1/21 (4.8) 1/21 (4.8) 100
      VZV & KSHV 1/21 (4.8) 1/21 (4.8) 100
      HSV-1 & HSV-2 & HCMV 1/21 (4.8) 1/21 (4.8) 100
      HSV-2 & VZV & HHV-7 & KSHV 1/21 (4.8) 1/21 (4.8) 100
      Negative 0/21(0) 0/21 (0) 100
      Total 21/21 (100) 21/21 (100) 100

      Table 2.  Evaluation of the single-tube nonuple qPCR assay for nine human herpesviruses and the reference gene GAPDH using 21 vesicular fluid samples.

    • Proportion in all samples (%) Proportion of positive samples (%)
      HHV single infection rate 25.9 60.3
      HHVs double infection rate 15.9 37
      HHV multiple infections rate 1.2 2.7
      Common HHV single infection rate
      EBV 11.2 26
      VZV 4.1 9.6
      CMV 3.5 8.2
      KSHV 2.3 5.5
      HSV-1 2.3 5.5
      HSV-2 1.7 4.1
      High proportion of HHV among multiple infections
      HSV-2 10.6 24.7
      EBV 10.6 24.7
      CMV 8.8 20.5
      VZV 1.8 4.1
      HHV-7 1.8 4.1
      KSHV 1.8 4.1
      HSV-1 0.6 1.4
      A total of 170 samples were tested

      Table 3.  Detection rates of various HHVs.