Virus	No. detected				Sensitivity	Specificity	PPV	NPV
Virus	M+R+	M+R-	M-R+	M-R-	(%)	(%)	(%)	(%)
RSV	46	92	6	294	33.3	98	88.5	76.2
FluA	4	32	2	400	11.1	99.5	66.7	92.6
hMPV	21	9	3	405	70	99.3	87.5	97.8
PIV3	19	7	5	407	73.1	98.9	79.2	98.4
H1N1-p^**	10	15	0	289	40	100	100	95.1
PIV1	12	6	3	417	66.7	99.3	80	98.6
hBoV	10	8	0	420	55.6	100	100	98.1
OC43	4	11	1	422	26.7	99.8	80	97.5
EnV	2	12	30	394	14.3	92.9	6.3	97
ADV	4	3	0	431	57.1	100	100	99.3
NL63	3	3	1	431	50	99.8	75	99.3
229E	3	2	1	432	60	99.8	75	99.5
FluB	2	2	0	434	50	100	100	99.5
PIV4	2	1	1	434	66.7	99.8	66.7	99.8
PIV2	0	3	0	435	NA	100	NA	99.3
HKU1	0	0	0	438	NA	NA	NA	NA
^* RhV was not evaluated as it was not detected by the monoplex real-time RT-PCR. PPV, positive predictive value; NPV, negative predictive value. ^** The Resplex Ⅱ Panel v2.0 was replaced by a ResPlex Ⅱ Plus Panel PRE to enhance H1N1-P coverage during the study. A total of 314 samples were tested by the ResPlex Ⅱ Plus Panel PRE version.

Table 1. The comparison of ResPlex (R) and monoplex real-time TaqMan RT-PCR (M) for 16 respiratory viruses ^*

Monoplex copies/mL	No. of ResPlex-PCR positive/No. of Monoplex PCR positive
Monoplex copies/mL	RSV (%)	FluA (%)	hMPV (%)	PIV3 (%)	H1N1-p (%)	PIV1 (%)	hBoV (%)
≥10⁶	6/9 (66.7)	1/1 (100)	3/4 (75.0)	2/3 (66.7)	4/5 (80.0)	0/0 (0)	9/11 (81.8)
1-9.9×10⁵	34/45 (75.5)	2/5 (40.0)	10/14 (71.4)	8/8 (100.0)	5/6 (83.3)	5/5 (100.0)	1/3 (33.3)
1-9.9×10⁴	5/38 (13.2)	1/7 (14.3)	6/9 (66.7)	4/5 (80.0)	1/6 (16.7)	6/7 (85.7)	0/2 (0)
1-9.9×10³	1/33 (3.0)	0/12 (0)	2/3 (66.7)	2/4 (50.0)	0/6 (0)	1/3 (33.3)	0/1 (0)
1-9.9×10²	0/13 (0)	0/11 (0)	0/0 (0)	3/6 (50.0)	0/2 (0)	0/3 (0)	0/1 (0)
Negative	6/300 (2.0)	2/402 (0.5)	3/408 (0.7)	5/412 (1.2)	0/289 (0)	3/420 (0.7)	0/420 (0)
z	-6.859	-5.119	-1.267	-1.011	-2.538	-1.068	-1.511
p	0.000	0.000	0.205	0.312	0.011	0.285	0.131

Table 2. Relationship of sensitivity of ResPlex PCR and viral loads determined by monoplex PCR

Virus	Resplex Ⅱ Panel No. of specimens				Sensitivity (%)	ResPlex Ⅱ Plus Panel PRE No. of specimens				Sensitivity (%)	χ² value	P value
Virus	M+R+	M+R-	M-R+	M-R-	Sensitivity (%)	M+R+	M+R-	M-R+	M-R-	Sensitivity (%)	χ² value	P value
RSV	14	25	3	82	35.9	32	67	3	212	32.3	0.161	0.688
FluA	2	2	2	118	50.0	2	30	0	282	6.3	6.891	0.053
hMPV	4	4	2	114	50.0	17	5	1	291	77.3	2.078	0.195
PIV3	6	0	2	116	100.0	13	7	3	291	65.0	2.874	0.146
PIV1	3	0	2	119	100.0	9	6	1	298	60.0	1.80	0.515
hBoV	5	1	0	118	83.3	5	7	0	302	41.7	2.813	0.152
^* R, ResPlex Ⅱ Panel; M, monoplex real-time TaqMan RT-PCR.

Table 3. Sensitivity of two kinds of ResPlex Ⅱ kit for common viruses ^*