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PDB 5Y6Z Data collectiona Space group P31 Cell dimensions a, b, c(Å) 93.7, 93.7, 167.6 α, β, γ(°) 90, 90, 120 Resolution (Å)b 46.0–2.50 (2.59–2.50) Rmerge 0.132 (0.372) I/σI 4.7 (2.1) Completeness (%) 99.5 (99.9) Redundancy 2.9 (3.0) Structure refinement Resolution (Å) 46.0–2.50 No. unique reflections 56, 671 Rwork/Rfreec(%) 21.8/25.9 No. atoms Protein/RNA 7247/1204 Ligand/Ion/Water 36/21/377 B-factors (Å2) Protein 44.5/55.9 Ligand/Ion/Water 48.6/56.2/44.5 R.m.s. deviations Bond lengths (Å) 0.008 Bond angles (°) 0.985 Ramachandran stat.d 93.6/6.0/0.2/0.2 Note: a One crystal was used for data collection. b Values in parentheses are for the highest-resolution shell. c 5% of data are taken for the Rfree set. d Values are in percentage and are for most favored, additionally allowed, generously allowed, and disallowed regions in Ramachandran plots, respectively. Table 1. X-ray diffraction data collection and structure refinement statistics
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PDB Description Reference 5Y6Z CV A16 RdRP EC, panels A-F This work 5F8G EV71 RdRP state 1 complex, panel B (top) Shu and Gong, 2016 5F8J EV71 RdRP state 4 complex, panel B (top) Shu and Gong, 2016 4K4X CV B3 RdRP state 1 complex, panel B (bottom) and panel C Gong et al., 2013 3N6L Apo EV71 RdRP (panel D) Wu et al., 2010 3DDK Apo CV B3 RdRP (panel D) Campagnola et al., 2008 1RA6 Apo PV RdRP (panel D) Thompson and Peersen, 2004 1XR7 Apo RV RdRP (panel D) Love et al., 2004 1U09 Apo FMDV RdRP (panel E) Ferrer-Orta et al., 2004 1C2P Apo HCV RdRP (panel E) Lesburg et al., 1999 3QID Apo NV RdRP (panel E) Lee et al., 2011 1KHV RHDV RdRP with a Lu3+ bound (panel F) Ng et al., 2002 1KHW RHDV RdRP with two Mn2+ bound (panel F) Ng et al., 2002 4NYZ EMCV RdRP with a glutamine bound (panel F) Vives-Adrian et al., 2014 Note: Abbreviations: CV-coxsackievirus; EV-Enterovirus; PV-poliovirus; RV-rhinovirus; FMDV-Foot-and-mouth disease virus; HCV-Hepatitis C virus; NV-Norovirus; RHDV-Rabbit hemorrhagic disease virus; EMCV-encephalomyocarditis virus. All virus names assigned as species by the International Committee on Taxonomy of Viruses (ICTV) are shown in italic and with the first letter capitalized. Table S1. PDB entries and related references in Figure 1.
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