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Bat species Pattern No. of tested samples (positive) Location Time (month/year) swab/faces/urine (CoV)(AdV)(PMV) Familiy Pteropodidae Cynopterus sphinx 1/0/0 1(0)(0)(0) 4 3/2016 Eonycteris spelaea 0/1/0 1(0)(0)(0) 2 5/2015 Familiy Rhinolophidae Rhinolophus pusillus 0/356/0 356(2)(0)(1) 2, 5 4, 5, 9, 10, 12/2015; 2/2016 Rhinolophus sinicus 0/0/2 2(0)(0)(0) 6 11/2015 Familiy Hipposideridae Hipposideros armiger 0/421/0 421(0)(4)(0) 2, 4, 6, 7 4, 6, 7, 8, 9, 10, 12/2015; 3/2016 Famliy Vespertilionidae Pipistrellus abramus 8/4/0 12(0)(2)(0) 3 4, 9/2015 Pipistrellus minus 13/0/0 13(0)(0)(0) 3 9/2015 Miniopterus schreibersii 78/0/0 78(24)(0)(1) 1 7/2015; 3/2016 Myotis ricketti 42/0/0 42(10)(16)(0) 1 7/2015; 3/2016 Tylonycteris pachypus 78/0/0 78(14)(0)(0) 4 4, 7/2015; 5/2016 Table 1. Samples collected and virus detection results
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Virus species Bat species and number of individuals in which viruses were detected R. pusillus H. armiger P. abramus M. schreibersii M. ricketti T. pachypus CoV HKU4 14 HKU7 1 1A/1B 2 23 HKU6 10 AdV Unclassified 4 2 16 PMV Unclassified 1 1 Co-infection 1A/Unclassified PMV 1 HKU6/Unclassified AdV 10 Note: Abbreviated species names: R. pusillus, Rhinolophus pusillus; H. armiger, Hipposideros armiger; P. abramus, Pipistrellus abramus; M. schreibersii, Miniopterus schreibersii; M. ricketti, Myotis ricketti; T. pachypus, Tylonycteris pachypus. Table 2. Coronaviruses, adenoviruses and paramyxoviruses detected in different bat species
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Strain Accession No. Host species BtTpCoV160079 BtTpCoV160081 BtTpCoV161028 Nucleotide sequence identity/Amino acid sequence identity 133/2005 DQ648794 Tylonycteris pachypus 97.4/97.6 97.4/97.6 93.6/97.9 GX2012 KJ473822 Tylonycteris pachypus 93.2/96.2 93.2/96.2 96.3/96.3 NeoCoV KC869678 Neoromicia capensis 63.0/60.3 63.2/60.5 63.1/60.4 EriCoV NC_022643 Erinaceus europaeus 62.3/57.5 62.2/57.4 62.4/57.2 SC2013 KJ473821 Vespertilio superans 68.2/70.6 68.6/70.8 68.6/71.2 HKU4 NC_009019 Tylonycteris pachypus 96.1/98.4 95.3/97.7 95.5/97.9 HKU5 NC_009020 Pipistrellus abramus 68.9/69.4 69.1/69.3 68.8/69.3 MERS-CoV KJ477102 Camel 67.5/67.2 67.8/67.4 67.5/67.4 MERS-CoV NC_019843 Human 67.4/67.5 67.8/67.6 67.5/67.7 Table 3. Comparison of S gene between HKU4-related strains detected in Macau and other Lineage C betacoronaviruses.
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