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Volume 37 Issue 1
February 2022
    Citation: Ying Tang, Yiqin Wang, Yuchang Li, Huai Zhao, Sen Zhang, Ying Zhang, Jing Li, Yuehong Chen, Xiaoyan Wu, Chengfeng Qin, Tao Jiang, Xiaoping Kang. An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2 .VIROLOGICA SINICA, 2022, 37(1) : 138-141.  http://dx.doi.org/10.1016/j.virs.2022.01.006
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    An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2

    • a Department of Virology, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing 100071, China

    • b State Key Laboratory of Pathogen and Biosecurity, AMMS, Beijing 100071, China

    • c Beijing University of Chemical Technology, Beijing 100029, China

    • d Technology Center of Erenhot Customs, Erenhot 011100, China

    • e Lifereal Biotech. Inc., Hangzhou 311100, China

    • f Anhui Medical University, Hefei 230032, China

    • Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a novel coronavirus that causes the outbreak of coronavirus disease 2019 (COVID-19) (Li et al., 2020a). Viral nucleic acid testing is the standard method for the laboratory diagnosis of COVID-19 (Wu et al., 2020a; Zhu et al., 2020). Currently, a variety of qPCR-based detection kits are used for laboratory-based detection and confirmation of SARS-CoV-2 infection (Corman et al., 2020; Hussein et al., 2020; Ruhan et al., 2020; Veyer et al., 2020). Conventional qPCR involves virus inactivation, nucleic acid extraction, and qPCR amplification procedures. Therefore, the process is complicated, which usually takes longer than 2 h, and requires biosafety laboratories and professional staff. Thus, qPCR is not suitable for use in field or medical units. To reduce the operation steps, automatic integrated qPCR detection systems that combine nucleic acid extraction and qPCR amplification in a sealed cartridge were developed to detect viruses in clinical samples (Li et al., 2020b). However, the detection time is still longer than 1 h. Therefore, rapid nucleic acid detection systems are needed to further improve the detection efficiency.
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      10.1016j.virs.2022.01.006-ESM.docx

      1. Behrmann O, Bachmann I, Spiegel M, Schramm M, Abd El Wahed A, Dobler G, Dame G, Hufert FT (2020) Rapid detection of SARS-CoV-2 by low volume real-yime single tube reverse transcription recombinase polymerase amplification using an exo probe with an internally linked quencher (Exo-IQ). Clin Chem 66:1047-1054.

      2. Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, Bleicker T, Brunink S, Schneider J, Schmidt ML, Mulders DG, Haagmans BL, van der Veer B, van den Brink S, Wijsman L, Goderski G, Romette JL, Ellis J, Zambon M, Peiris M, Goossens H, Reusken C, Koopmans MP, Drosten C (2020) Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill 25:2000045.

      3. Hussein HA, Hassan RYA, Chino M, Febbraio F (2020) Point-of-Care diagnostics of COVID-19: from current work to future perspectives. Sensors (Basel) 20:4289.

      4. Jia J, Li Y, Wu X, Zhang S, Hu Y, Li J, Jiang T, Kang X (2019) Reverse transcription recombinase polymerase amplification assays for rapid detection of tick-borne encephalitis virus infection. Virol Sin 34:338-341.

      5. Li Q, Guan X, Wu P, Wang X, Zhou L, Tong Y, Ren R, Leung KSM, Lau EHY, Wong JY, Xing X, Xiang N, Wu Y, Li C, Chen Q, Li D, Liu T, Zhao J, Liu M, Tu W, Chen C, Jin L, Yang R, Wang Q, Zhou S, Wang R, Liu H, Luo Y, Liu Y, Shao G, Li H, Tao Z, Yang Y, Deng Z, Liu B, Ma Z, Zhang Y, Shi G, Lam TTY, Wu JT, Gao GF, Cowling BJ, Yang B, Leung GM, Feng Z (2020a) Early transmission dynamics in Wuhan, China, of novel coronavirus-infected pneumonia. N Engl J Med 382:1199-1207.

      6. Li Y, Li J, Zhang Y, Dai L, Li L, Liu J, Zhang S, Wu X, Hu Y, Qin C, Jiang T, Kang X (2020b) Development of an automatic integrated gene detection system for novel severe acute respiratory syndrome-related coronavirus (SARS-CoV2). Emerg Microbes Infect 9:1489-1496.

      7. Piepenburg O, Williams CH, Stemple DL, Armes NA (2006) DNA detection using recombination proteins. PLoS Biol 4:e204.

      8. Ruhan A, Wang H, Wang W, Tan W (2020) Summary of the detection kits for SARS-CoV-2 approved by the National Medical Products Administration of China and their application for diagnosis of COVID-19. Virol Sin 35:699-712.

      9. Veyer D, Kerneis S, Poulet G, Wack M, Robillard N, Taly V, L'Honneur AS, Rozenberg F, Laurent-Puig P, Belec L, Hadjadj J, Terrier B, Pere H (2020) Highly sensitive quantification of plasma SARS-CoV-2 RNA shelds light on its potential clinical value. Clin Infect Dis :ciaa1196.

      10. Wang J, Liu L, Wang J, Sun X, Yuan W (2017) Recombinase polymerase amplification assay-A simple, fast and cost-effective alternative to real time PCR for specific detection of feline herpesvirus-1. PLoS One 12:e0166903.

      11. Wu F, Zhao S, Yu B, Chen YM, Wang W, Song ZG, Hu Y, Tao ZW, Tian JH, Pei YY, Yuan ML, Zhang YL, Dai FH, Liu Y, Wang QM, Zheng JJ, Xu L, Holmes EC, Zhang YZ (2020a) A new coronavirus associated with human respiratory disease in China. Nature 579:265-269.

      12. Wu T, Ge Y, Zhao K, Zhu X, Chen Y, Wu B, Zhu F, Zhu B, Cui L (2020b) A reverse-transcription recombinase-aided amplification assay for the rapid detection of N gene of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). Virology 549:1-4. .

      13. Xu L, Liang W, Yang X, Wen Y, Li L, Yang Z, Li Y, Deng M, Lu Q, Ding M, Ren S, Sun J, Zuo X, Wang L, Cao C, Hu J, Liu G, Fa C (2020) SARS-CoV-2 RNA reference materials. Chin Sci Bull 65: 2623-2370.

      14. Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, Zhao X, Huang B, Shi W, Lu R, Niu P, Zhan F, Ma X, Wang D, Xu W, Wu G, Gao GF, Tan W; China Novel Coronavirus Investigating and Research Team (2020) A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med 382:727-733

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      An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2

        Corresponding author: Tao Jiang, jiangtao@bmi.ac.cn
        Corresponding author: Xiaoping Kang, kangxiaoping@163.com
      • a Department of Virology, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing 100071, China
      • b State Key Laboratory of Pathogen and Biosecurity, AMMS, Beijing 100071, China
      • c Beijing University of Chemical Technology, Beijing 100029, China
      • d Technology Center of Erenhot Customs, Erenhot 011100, China
      • e Lifereal Biotech. Inc., Hangzhou 311100, China
      • f Anhui Medical University, Hefei 230032, China
      • Received Date: 31 May 2021
      • Accepted Date: 30 September 2021

      Abstract: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a novel coronavirus that causes the outbreak of coronavirus disease 2019 (COVID-19) (Li et al., 2020a). Viral nucleic acid testing is the standard method for the laboratory diagnosis of COVID-19 (Wu et al., 2020a; Zhu et al., 2020). Currently, a variety of qPCR-based detection kits are used for laboratory-based detection and confirmation of SARS-CoV-2 infection (Corman et al., 2020; Hussein et al., 2020; Ruhan et al., 2020; Veyer et al., 2020). Conventional qPCR involves virus inactivation, nucleic acid extraction, and qPCR amplification procedures. Therefore, the process is complicated, which usually takes longer than 2 h, and requires biosafety laboratories and professional staff. Thus, qPCR is not suitable for use in field or medical units. To reduce the operation steps, automatic integrated qPCR detection systems that combine nucleic acid extraction and qPCR amplification in a sealed cartridge were developed to detect viruses in clinical samples (Li et al., 2020b). However, the detection time is still longer than 1 h. Therefore, rapid nucleic acid detection systems are needed to further improve the detection efficiency.

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