Fish ACE2 is not susceptible to SARS-CoV-2

Shi-Zhe Xie; Mei-Qin Liu; Ren-Di Jiang; Hao-Feng Lin; Wei Zhang; Bei Li; Jia Su; Fei Ke; Qi-Ya Zhang; Zheng-Li Shi; Xing-Lou Yang

doi:10.1016/j.virs.2022.01.020

February 2022

Citation: Shi-Zhe Xie, Mei-Qin Liu, Ren-Di Jiang, Hao-Feng Lin, Wei Zhang, Bei Li, Jia Su, Fei Ke, Qi-Ya Zhang, Zheng-Li Shi, Xing-Lou Yang. Fish ACE2 is not susceptible to SARS-CoV-2 .VIROLOGICA SINICA, 2022, 37(1) : 142-144. http://dx.doi.org/10.1016/j.virs.2022.01.020

Fish ACE2 is not susceptible to SARS-CoV-2

Shi-Zhe Xie ^a,b ,
Mei-Qin Liu ^a,b ,
Ren-Di Jiang ^a,b ,
Hao-Feng Lin ^a,b ,
Wei Zhang ^a ,
Bei Li ^a ,
Jia Su ^a,b ,
Fei Ke ^c ,
Qi-Ya Zhang ^c ,
Zheng-Li Shi ^a ,
Xing-Lou Yang ^{a
,,}

a CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China
b University of Chinese Academy of Sciences, Beijing, 100049, China

Corresponding author: Xing-Lou Yang, yangxl@wh.iov.cn
Received Date: 26 August 2021
Accepted Date: 12 January 2022
Available online: 20 January 2022

Abstract

The ongoing pandemic of coronavirus disease 2019 (COVID-19) has reshaped our daily life and caused > 4 million deaths worldwide (https://covid19.who.int/). Although lockdown and vaccination have improved the situation in many countries, imported cases and sporadic outbreaks pose a constant stress to the prevention and control of COVID-19. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiologic agent responsible for COVID-19, has a positive-sense single-stranded RNA genome of 30 kb (Coronaviridae Study Group of the International Committee on Taxonomy of Viruses, 2020). We and other groups have demonstrated that the SARS-CoV-2 could use the angiotensin-converting enzyme 2 (ACE2) as cell receptor, including orthologs of a broad range of animal species such as human, bats, ferrets, pigs, cats, and dogs (Hoffmann et al., 2020; Zhou et al., 2020; Liu et al., 2021). Although the evolutionary origin of SARS-CoV-2 can be linked to the discoveries of diverse coronaviruses related to SARS-CoV-2 in wild animals such as bats (Zhou et al., 2020; Wacharapluesadee et al., 2021) and pangolins (Liu et al., 2019; Lam et al., 2020), the direct origin of SARS-CoV-2 in humans remains unknown. In China, several sporadic outbreaks of COVID-19 in 2020 were linked to food in cold chain sold at trade markets, including salmon meat (http://www.nhc.gov.cn/xcs/yqtb/list_gzbd.shtml) (Yang et al., 2020). The detection of SARS-CoV-2 RNA on the surface of frozen meat for as long as 20 days has also been reported (Feng et al., 2021). A concern regarding the potential role of fish in SARS-CoV-2 transmission has also been raised. Therefore, we investigated the susceptibility of fish ACE2 to SARS-CoV-2.

Electronic Supplementary Material

10.1016j.virs.2022.01.020-ESM1.docx
10.1016j.virs.2022.01.020-ESM3.pdf
10.1016j.virs.2022.01.020-ESM2.pdf

References
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Fish ACE2 is not susceptible to SARS-CoV-2

Corresponding author: Xing-Lou Yang, yangxl@wh.iov.cn

a CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

b University of Chinese Academy of Sciences, Beijing, 100049, China

Received Date: 26 August 2021

Accepted Date: 12 January 2022

Abstract: The ongoing pandemic of coronavirus disease 2019 (COVID-19) has reshaped our daily life and caused > 4 million deaths worldwide (https://covid19.who.int/). Although lockdown and vaccination have improved the situation in many countries, imported cases and sporadic outbreaks pose a constant stress to the prevention and control of COVID-19. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiologic agent responsible for COVID-19, has a positive-sense single-stranded RNA genome of 30 kb (Coronaviridae Study Group of the International Committee on Taxonomy of Viruses, 2020). We and other groups have demonstrated that the SARS-CoV-2 could use the angiotensin-converting enzyme 2 (ACE2) as cell receptor, including orthologs of a broad range of animal species such as human, bats, ferrets, pigs, cats, and dogs (Hoffmann et al., 2020; Zhou et al., 2020; Liu et al., 2021). Although the evolutionary origin of SARS-CoV-2 can be linked to the discoveries of diverse coronaviruses related to SARS-CoV-2 in wild animals such as bats (Zhou et al., 2020; Wacharapluesadee et al., 2021) and pangolins (Liu et al., 2019; Lam et al., 2020), the direct origin of SARS-CoV-2 in humans remains unknown. In China, several sporadic outbreaks of COVID-19 in 2020 were linked to food in cold chain sold at trade markets, including salmon meat (http://www.nhc.gov.cn/xcs/yqtb/list_gzbd.shtml) (Yang et al., 2020). The detection of SARS-CoV-2 RNA on the surface of frozen meat for as long as 20 days has also been reported (Feng et al., 2021). A concern regarding the potential role of fish in SARS-CoV-2 transmission has also been raised. Therefore, we investigated the susceptibility of fish ACE2 to SARS-CoV-2.

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