Citation: Haojie Ren, Xiaoguang Yan, Lintao Liu, Yixuan Zhang, Qianqian Li, Xiumei Li, Hui Hu. Identification of two novel B-cell epitopes on the nucleocapsid protein of porcine deltacoronavirus .VIROLOGICA SINICA, 2022, 37(2) : 303-306.  http://dx.doi.org/10.1016/j.virs.2022.01.025

Identification of two novel B-cell epitopes on the nucleocapsid protein of porcine deltacoronavirus

  • Corresponding author: Hui Hu, huhui2001@163.com
  • Received Date: 26 May 2021
    Accepted Date: 14 January 2022
    Available online: 24 January 2022
  • Highlights
    1. Two monoclonal antibodies against newly emerged porcine deltacoronavirus nucleocapsid protein were prepared.
    2. The epitopes that these two monoclonal antibodies recognized on nucleocapsid protein were identified.
    3. The monoclonal antibody 6B7 recognized a linear epitope of N protein, while the 7F8 recognized a conformational epitope.
    4. Conservation of the identified epitopes between different coronaviruses was analyzed.

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  • 10.1016j.virs.2022.01.025-ESM.docx
    1. Dinesh, D.C., Chalupska, D., Silhan, J., Koutna, E., Nencka, R., Veverka, V., Boura, E., 2020. Structural basis of rna recognition by the sars-cov-2 nucleocapsid phosphoprotein. PLoS Pathog. 16, e1009100.

    2. Jung, K., Hu, H., Saif, L.J., 2016. Porcine deltacoronavirus infection: etiology, cell culture for virus isolation and propagation, molecular epidemiology and pathogenesis. Virus Res. 226, 50–59.

    3. Korn, S.M., Lambertz, R., Fürtig, B., Hengesbach, M., Löhr, F., Richter, C., Schwalbe, H., Weigand, J.E., Wohnert, J., Schlundt, A., H, C., 2020. N backbone chemical shift assignments of the c-terminal dimerization domain of SARS-CoV-2 nucleocapsid protein. Biomol NMR Assign 15, 129–135.

    4. Tseng, Y.Y., Liao, G.R., Lien, A., Hsu, W.L., 2021. Current concepts in the development of therapeutics against human and animal coronavirus diseases by targeting np. Comput. Struct. Biotechnol. J. 19, 1072–1080.

    5. Van Elslande, J., Oyaert, M., Ailliet, S., Van Ranst, M., Lorent, N., Vande Weygaerde, Y., André, E., Lagrou, K., Vandendriessche, S., Vermeersch, P., 2021. Longitudinal follow-up of igg anti-nucleocapsid antibodies in sars-cov-2 infected patients up to eight months after infection. J. Clin. Virol. 136, 104765.

    6. Wurm, T., Chen, H., Hodgson, T., Britton, P., Brooks, G., Hiscox, J.A., 2001. Localization to the nucleolus is a common feature of coronavirus nucleoproteins, and the protein may disrupt host cell division. J. Virol. 75, 9345–9356.

    7. Xu, X., Zhang, H., Zhang, Q., Huang, Y., Dong, J., Liang, Y., Liu, H.J., Tong, D., 2013. Porcine epidemic diarrhea virus n protein prolongs s-phase cell cycle, induces endoplasmic reticulum stress, and up-regulates interleukin-8 expression. Vet. Microbiol. 164, 212–221.

    8. Yoo, D., Wootton, S.K., Li, G., Song, C., Rowland, R.R., 2003. Colocalization and interaction of the porcine arterivirus nucleocapsid protein with the small nucleolar rna-associated protein fibrillarin. J. Virol. 77, 12173–12183.

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    Identification of two novel B-cell epitopes on the nucleocapsid protein of porcine deltacoronavirus

      Corresponding author: Hui Hu, huhui2001@163.com
    • a College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China
    • b Key Laboratory for Animal-derived Food Safety of Henan Province, Zhengzhou, 450002, China
    • c Luoyang Laipson Information Technology Co., Ltd, Luoyang, 471026, China

    Abstract: Highlights
    1. Two monoclonal antibodies against newly emerged porcine deltacoronavirus nucleocapsid protein were prepared.
    2. The epitopes that these two monoclonal antibodies recognized on nucleocapsid protein were identified.
    3. The monoclonal antibody 6B7 recognized a linear epitope of N protein, while the 7F8 recognized a conformational epitope.
    4. Conservation of the identified epitopes between different coronaviruses was analyzed.

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