Citation: Lifang Song, Bopei Cui, Jinghuan Yang, Xiaotian Hao, Xujia Yan, Jialu Zhang, Dong Liu, Ziyang Song, Qian Wang, Qunying Mao, Zhenglun Liang. Construction and verification of an infectious cDNA clone of coxsackievirus B5 .VIROLOGICA SINICA, 2022, 37(3) : 469-471.  http://dx.doi.org/10.1016/j.virs.2022.03.005

Construction and verification of an infectious cDNA clone of coxsackievirus B5

  • Corresponding author: Qunying Mao, maoqunying@126.com
    Zhenglun Liang, lzhenglun@126.com
  • Received Date: 07 September 2021
    Accepted Date: 07 March 2022
    Available online: 11 March 2022
  • Highlights:
    · An infectious cDNA clone of CV-B5 was constructed.
    · The rescued and parental virus possessed similar biological characteristics.
    · The virulence of the rescued virus was similiar to that of the parental virus.
    · Viral distribution and tissue tropism of those two viruses were in agreement.

  • 加载中
  • 10.1016j.virs.2022.03.005-ESM.docx
    1. Chen, Y., Yang, S., Yang, H., Lin, T., Hsieh, Y., Arthur Huang, K., Kuo, C., Chiu, C., Huang, Y., Chu, S., Chen, J., 2020. Clinical characteristics of echovirus 11 and coxsackievirus B5 infections in Taiwanese children requiring hospitalization. J. Microbiol. Immunol. Infect. S1684-1182, 30149-30153.

    2. Ding, L., Brown, D., Glass, J., 2021. Rescue of infectious sindbis virus by yeast spheroplast-mammalian cell fusion. Viruses 13, 603.

    3. Duan, X., Chen, Z., Li, X., Ping, Y., Lu, L., 2021. Virus shedding in patients with hand, foot and mouth disease induced by EV71, CA16 or CA6:systematic review and metaanalysis. Pediatr. Infect. Dis. J. 40, 289-294.

    4. Françozo, M., Costa, F., Guerra-Gomes, I., Silva, J., Sesti-Costa, R., 2019. Dendritic cells and regulatory T cells expressing CCR4 provide resistance to coxsackievirus B5-induced pancreatitis. Sci. Rep. 9, 14766.

    5. Gao, F., Bian, L., Hao, X., Hu, Y., Yao, X., Sun, S., Chen, P., Yang, C., Du, R., Li, J., Zhu, F., Mao, Q., Liang, Z., 2018. Seroepidemiology of coxsackievirus B5 in infants and children in Jiangsu province, China. Hum Vaccin Immunother 14, 74-80.

    6. Hyoty, H., Leon, F., Knip, M., 2018. Developing a vaccine for type 1 diabetes by targeting coxsackievirus B. Expert Rev. Vaccines 17, 1071-1083.

    7. Hao, X., Song, S., Zhong, Q., Jamal-U-Ddin, H., Guo, J., Wu, Y., 2021. Rescue of an infection clone of barley yellow dwarf virus -GAV. Phytopathology 111, 2383-2391.

    8. Li, P., Yao, C., Wang, T., Wu, T., Yi, W., Zheng, Y., Miao, Y., Sun, J., Tan, Z., Liu, Y., Zhang, X., Wang, H., Zheng, Z., 2021. Recovery of a far-eastern strain of tick-borne encephalitis virus with a full-length infectious cDNA clone. Virol. Sin. 36, 1375-1386.

    9. Liu, Q., Dan, H., Zhao, X., Chen, H., Chen, Y., Zhang, N., Mo, Z., Liu, H., 2019. Construction and characterization of an infectious cDNA clone of coxsackievirus A 10. Virol. J. 16, 98.

    10. Ma, Y., Hao, S., Sun, L., Li, J., Qiao, Q., Gao, F., Zhao, L., Yu, X., Wang, Z., Wen, H., 2015. Construction and characterization of infectious cDNA clones of enterovirus 71(EV71). Virol. Sin. 30, 305-308.

    11. Mao, Q., Hao, X., Hu, Y., Du, R., Lang, S., Bian, L., Gao, F., Yang, C., Cui, B., Zhu, F., Shen, L., Liang, Z., 2018. A neonatal mouse model of central nervous system infections caused by Coxsackievirus B5. Emerg. Microb. Infect. 7, 185.

    12. Pan, M., Gao, S., Zhou, Z., Zhang, K., Liu, S., Wang, Z., Wang, T., 2018. A reverse genetics system for enterovirus D68 using human RNA polymerase I. Virus Gene. 54, 484-492.

    13. Sciandra, I., Falasca, F., Maida, P., 2020. Seroprevalence of group B coxsackieviruses:retrospective study in an Italian population. J. Med. Virol. 92, 3138-3143.

    14. Wang, M., Yan, J., Zhu, L., Wang, M., Liu, L., Yu, R., Chen, M., Xun, J., Zhang, Y., Yi, Z., Zhang, S., 2020. The establishment of infectious clone and single round infectious particles for coxsackievirus A10. Virol. Sin. 35, 426-435.

    15. Yang, L., Li, S., Liu, Y., Hou, W., Lin, Q., Zhao, H., Xu, L., He, D., Ye, X., Zhu, H., Cheng, T., Xia, N., 2015. Construction and characterization of an infectious clone of coxsackievirus A6 that showed high virulence in neonatal mice. Virus Res. 210, 165-168.

    16. Zaoutis, T., Klein, J., 1998. Enterovirus infections. Pediatr. Rev. 19, 183-191.

  • 加载中

Article Metrics

Article views(5730) PDF downloads(37) Cited by()

Related
Proportional views

    Construction and verification of an infectious cDNA clone of coxsackievirus B5

      Corresponding author: Qunying Mao, maoqunying@126.com
      Corresponding author: Zhenglun Liang, lzhenglun@126.com
    • a National Institute for Food and Drug Control, Beijing 102629, China;

    Abstract: Highlights:
    · An infectious cDNA clone of CV-B5 was constructed.
    · The rescued and parental virus possessed similar biological characteristics.
    · The virulence of the rescued virus was similiar to that of the parental virus.
    · Viral distribution and tissue tropism of those two viruses were in agreement.

    Reference (16) Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return