New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq

Mengyuan Chen; Shuizhen He; Hualong Xiong; Dongxu Zhang; Shaojuan Wang; Wangheng Hou; Xiaomei Zhu; Jin Wang; Yang Huang; Congming Hong; Yubin Wu; Ruoyao Qi; Tianying Zhang; Quan Yuan; Tingdong Li; Yixin Chen; Shiyin Zhang; Shengxiang Ge; Jun Zhang; Ningshao Xia

doi:10.1016/j.virs.2022.07.001

October 2022

Citation: Mengyuan Chen, Shuizhen He, Hualong Xiong, Dongxu Zhang, Shaojuan Wang, Wangheng Hou, Xiaomei Zhu, Jin Wang, Yang Huang, Congming Hong, Yubin Wu, Ruoyao Qi, Tianying Zhang, Quan Yuan, Tingdong Li, Yixin Chen, Shiyin Zhang, Shengxiang Ge, Jun Zhang, Ningshao Xia. New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq .VIROLOGICA SINICA, 2022, 37(5) : 758-761. http://dx.doi.org/10.1016/j.virs.2022.07.001

New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq

a State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, South Xiang'an Road, Xiamen, 361102, China;
b Xiamen Haicang Hospital, Haiyu Road, Xiamen, 361026, China;
c School of Life Sciences, Xiamen University, South Xiang'an Road, Xiamen, 361102, China

Corresponding author: Shiyin Zhang, zhangshiyin@xmu.edu.cn
Shengxiang Ge, sxge@xmu.edu.cn
Received Date: 28 July 2021
Accepted Date: 30 June 2022
Available online: 05 July 2022

Abstract

Highlights
1 A peptide Spep-1 targeting S2 of SARS-CoV-2 spike protein was selected by PhIP-Seq.
2 Spep-1 showed nanomolar affinity and high specificity to spike protein.
3 S-1 based immunoassay can detect femtomolar spike antigen in spiked serum samples.
4 Spep-1 can be used in future on S2 recognition, virus tracing and drug delivery.

Electronic Supplementary Material

10.1016j.virs.2022.07.001-ESM.docx
References
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New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq

Corresponding author: Shiyin Zhang, zhangshiyin@xmu.edu.cn

Corresponding author: Shengxiang Ge, sxge@xmu.edu.cn

a State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, South Xiang'an Road, Xiamen, 361102, China;

b Xiamen Haicang Hospital, Haiyu Road, Xiamen, 361026, China;

c School of Life Sciences, Xiamen University, South Xiang'an Road, Xiamen, 361102, China

Received Date: 28 July 2021

Accepted Date: 30 June 2022

Abstract: Highlights
1 A peptide Spep-1 targeting S2 of SARS-CoV-2 spike protein was selected by PhIP-Seq.
2 Spep-1 showed nanomolar affinity and high specificity to spike protein.
3 S-1 based immunoassay can detect femtomolar spike antigen in spiked serum samples.
4 Spep-1 can be used in future on S2 recognition, virus tracing and drug delivery.

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New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq

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