Citation: Kaw Bing Chua, Qimei Ng, Tao Meng, Qiang Jia. Development of stable, cold-adapted, temperature-sensitive/conditional lethal chimeric enterovirus A71 and coxsackievirus A16 .VIROLOGICA SINICA, 2022, 37(5) : 769-773.  http://dx.doi.org/10.1016/j.virs.2022.08.001

Development of stable, cold-adapted, temperature-sensitive/conditional lethal chimeric enterovirus A71 and coxsackievirus A16

  • Corresponding author: Kaw Bing Chua, chuakb@tll.org.sg
  • Received Date: 17 August 2021
    Accepted Date: 02 August 2022
    Available online: 11 August 2022
  • Highlights
    1 A stable EV-A71 virus vector was created to generate chimeric enterovirus strains expressing capsid protein genes of EV-A71 subgenogroup C5 and CA16.
    2 Phenotypic and genetic stability of the generated chimeric EV-A71 and CA16 were analyzed.
    3 The amino acids at the cleavage site between VP1 and 2A is crucial for stability.

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  • 10.1016j.virs.2022.08.001-ESM.docx
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    14. Lum, L.C.S., Wong, K.T., Lam, S.K., Chua, K.B., Goh, A.Y.T., Lim, W.L., Ong, B.B., Paul, G., AbuBakar, S., Lambert, M., 1998. Fatal enterovirus 71 encephalitis. J. Pediatr. 133, 795-798.

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    21. Zeng, M., El Khatib, N.F., Tu, S., Ren, P., Xu, S., Zhu, Q., Mo, X., Pu, D., Wang, X., Altmeyer, R., 2012. Seroepidemiology of enterovirus 71 infection prior to the 2011 season in children in Shanghai. J. Clin. Virol. 53, 285-289.

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    Development of stable, cold-adapted, temperature-sensitive/conditional lethal chimeric enterovirus A71 and coxsackievirus A16

      Corresponding author: Kaw Bing Chua, chuakb@tll.org.sg
    • Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604, Singapore

    Abstract: Highlights
    1 A stable EV-A71 virus vector was created to generate chimeric enterovirus strains expressing capsid protein genes of EV-A71 subgenogroup C5 and CA16.
    2 Phenotypic and genetic stability of the generated chimeric EV-A71 and CA16 were analyzed.
    3 The amino acids at the cleavage site between VP1 and 2A is crucial for stability.

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