Roles of the functional domains and conserved residues of the severe fever with thrombocytopenia syndrome virus L protein provide insights into the viral RNA transcription/replication mechanism

Zhiyun Yin; Fuli Ren; Min Zhou; Shengyao Chen; Yali Deng; Sijing Hu; Fei Deng; Shu Shen; Junming Shi

doi:10.1016/j.virs.2022.08.009

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Volume 37 Issue 6

December 2022

Citation: Zhiyun Yin, Fuli Ren, Min Zhou, Shengyao Chen, Yali Deng, Sijing Hu, Fei Deng, Shu Shen, Junming Shi. Roles of the functional domains and conserved residues of the severe fever with thrombocytopenia syndrome virus L protein provide insights into the viral RNA transcription/replication mechanism .VIROLOGICA SINICA, 2022, 37(6) : 946-949. http://dx.doi.org/10.1016/j.virs.2022.08.009

Roles of the functional domains and conserved residues of the severe fever with thrombocytopenia syndrome virus L protein provide insights into the viral RNA transcription/replication mechanism

Zhiyun Yin ^a,b ,
Fuli Ren ^a,c ,
Min Zhou ^a ,
Shengyao Chen ^a ,
Yali Deng ^a ,
Sijing Hu ^a ,
Fei Deng ^a ,
Shu Shen ^{a
,,} ,
Junming Shi ^{a
,,}

a National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China;
b Academic Affairs Office of Cangzhou Jiaotong University, Cangzhou, 061199, China;
c Center for Translational Medicine, Jinyintan Hospital, Wuhan, 430023, China

Corresponding author: Shu Shen, shenshu@wh.iov.cn
Junming Shi, sjm@wh.iov.cn
Received Date: 17 June 2022
Accepted Date: 26 August 2022
Available online: 03 September 2022

Abstract

Highlights
1. All the domains of SFTSV-L protein were required for its function in viral RNA replication/transcription.
2. The influence of twelve conserved residues speculated basing on the atomic model of SFTSV-L were evaluated using mini-genome system, and nine of which showed significant roles in the function of L protein.
3. Nine key residues of SFTSV-L were strictly conserved among emerging related phleboviruses, suggesting L protein may be a promising broad spectrum drug target of phleboviruses.

Electronic Supplementary Material

10.1016j.virs.2022.08.009-ESM.docx
References
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Roles of the functional domains and conserved residues of the severe fever with thrombocytopenia syndrome virus L protein provide insights into the viral RNA transcription/replication mechanism

Corresponding author: Shu Shen, shenshu@wh.iov.cn

Corresponding author: Junming Shi, sjm@wh.iov.cn

a National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China;
b Academic Affairs Office of Cangzhou Jiaotong University, Cangzhou, 061199, China;
c Center for Translational Medicine, Jinyintan Hospital, Wuhan, 430023, China

Received Date: 17 June 2022
Accepted Date: 26 August 2022

Abstract: Highlights
1. All the domains of SFTSV-L protein were required for its function in viral RNA replication/transcription.
2. The influence of twelve conserved residues speculated basing on the atomic model of SFTSV-L were evaluated using mini-genome system, and nine of which showed significant roles in the function of L protein.
3. Nine key residues of SFTSV-L were strictly conserved among emerging related phleboviruses, suggesting L protein may be a promising broad spectrum drug target of phleboviruses.