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Volume 38 Issue 1
February 2023
    Citation: Yuchang Li, Yanhong Gao, Ying Tang, Jing Li, Sen Zhang, Tao Jiang, Xiaoping Kang. Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus .VIROLOGICA SINICA, 2023, 38(1) : 165-170.  http://dx.doi.org/10.1016/j.virs.2022.12.001
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    Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus

    • a. State Key Laboratory of Pathogen and Biosecurity, The Academy of Military Medical Science, Institute of Microbiology and Epidemiology, Beijing, 100071, China;

    • b. Laboratory Department of the First Medical Center, Chinese PLA General Hospital, Beijing, 100850, China

    • Highlights
      1. Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection.
      2. The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA,
      3. No cross-reaction was found in 14 rash and fever-associated viruses.
      4. The two RPA methods developed are simple and rapid, making them potentially useful for detection of MPXV in the field/clinic.
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    • Electronic Supplementary Material

      10.1016j.virs.2022.12.001-ESM.docx

      1. Adler, H., Gould, S., Hine, P., Snell, L.B., Wong, W., Houlihan, C.F., Osborne, J.C., Rampling, T., Beadsworth, M.B., Duncan, C.J., Dunning, J., Fletcher, T.E., Hunter, E.R., Jacobs, M., Khoo, S.H., Newsholme, W., Porter, D., Porter, R.J., Ratcliffe, L., Schmid, M.L., Semple, M.G., Tunbridge, A.J., Wingfield, T., Price, N.M., NHS England High Consequence Infectious Diseases (Airborne) Network, 2022.Clinical features and management of human monkeypox:a retrospective observational study in the UK. Lancet Infect. Dis. 22, 1153-1162.

      2. Bunge, E.M., Hoet, B., Chen, L., Lienert, F., Weidenthaler, H., Baer, L.R., Steffen, R., 2022.The changing epidemiology of human monkeypox-A potential threat? A systematic review. PLoS Neglected Trop. Dis. 16, e0010141.

      3. Davi, S.D., Kissenkötter, J., Faye, M., Böhlken-Fascher, S., Stahl-Hennig, C., Faye, O., Faye, O., Sall, A.A., Weidmann, M., Ademowo, O.G., Hufert, F.T., Czerny, C.P., Abd El Wahed, A., 2019. Recombinase polymerase amplification assay for rapid detection of Monkeypox virus. Diagn. Microbiol. Infect. Dis. 95, 41-45.

      4. Erez, N., Achdout, H., Milrot, E., Schwartz, Y., Wiener-Well, Y., Paran, N., Politi, B., Tamir, H., Israely, T., Weiss, S., Beth-Din, A., Shifman, O., Israeli, O., Yitzhaki, S., Shapira, S.C., Melamed, S., Schwartz, E., 2019. Diagnosis of imported monkeypox, Israel, 2018. Emerg. Infect. Dis. 25, 980-983.

      5. Jia, J., Li, Y., Wu, X., Zhang, S., Hu, Y., Li, J., Jiang, T., Kang, X., 2019. Reverse transcription recombinase polymerase amplification assays for rapid detection of tick-borne encephalitis virus infection. Virol. Sin. 34, 338-341.

      6. Mauldin, M.R., McCollum, A.M., Nakazawa, Y.J., Mandra, A., Whitehouse, E.R., Davidson, W., Zhao, H., Gao, J., Li, Y., Doty, J., Yinka-Ogunleye, A., Akinpelu, A., Aruna, O., Naidoo, D., Lewandowski, K., Afrough, B., Graham, V., Aarons, E., Hewson, R., Vipond, R., Dunning, J., Chand, M., Brown, C., Cohen-Gihon, I., Erez, N., Shifman, O., Israeli, O., Sharon, M., Schwartz, E., Beth-Din, A., Zvi, A., Mak, T.M., Ng, Y.K., Cui, L., Lin, R.T.P., Olson, V.A., Brooks, T., Paran, N., Ihekweazu, C., Reynolds, M.G., 2022. Exportation of monkeypox virus from the African Continent.J. Infect. Dis. 225, 1367-1376.

      7. Miura, F., van Ewijk, C.E., Backer, J.A., Xiridou, M., Franz, E., Op de Coul, E., Brandwagt, D., van Cleef, B., van Rijckevorsel, G., Swaan, C., van den Hof, S.,Wallinga, J., 2022. Estimated incubation period for monkeypox cases confirmed in The Netherlands, May 2022. Euro Surveill. 27, 2200448.

      8. Stern, D., Olson, V.A., Smith, S.K., Pietraszczyk, M., Miller, L., Miethe, P., Dorner, B.G., Nitsche, A., 2016. Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration. Virol. J. 13, 207.

      9. Tang, Y., Wang, Y., Li, Y., Zhao, H., Zhang, S., Zhang, Y., Li, J., Chen, Y., Wu, X., Qin, C., Jiang, T., Kang, X., 2022. An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2. Virol. Sin. 37, 138-141.

      10. The International Committee on Taxonomy of Viruses (ICTV), 2022. Virus Taxonomy:2020 Release. https://talk.ictvonline.org/taxonomy.

      11. Thornhill, J.P., Barkati, S., Walmsley, S., Rockstroh, J., Antinori, A., Harrison, L.B., Palich, R., Nori, A., Reeves, I., Habibi, M.S., Apea, V., Boesecke, C., Vandekerckhove, L., Yakubovsky, M., Sendagorta, E., Blanco, J.L., Florence, E., Moschese, D., Maltez, F.M., Goorhuis, A., Pourcher, V., Migaud, P., Noe, S., Pintado, C., Maggi, F., Hansen, A.E., Hoffmann, C., Lezama, J.I., Mussini, C., Cattelan, A., Makofane, K., Tan, D., Nozza, S., Nemeth, J., Klein, M.B., Orkin, C.M., SHARE-net Clinical Group, 2022. Monkeypox virus infection in humans across 16 countries-April-June 2022. N. Engl. J. Med. 387, 679-691.

      12. Wang, S., Li, Y., Zhang, F., Jiang, N., Zhuang, Q., Hou, G., Jiang, L., Yu, J., Yu, X., Liu, H., Zhao, C., Yuan, L., Huang, B., Wang, K., 2022. Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus. Virol. J. 19, 129.

      13. World Health Organization (WHO), 2022a. Multi-country monkeypox outbreak:situation update. https://www.who.int/emergencies/disease-outbreak-news/item/2022-DON393.

      14. World Health Organization (WHO), 2022b. Surveillance, case investigation and contact tracing for monkeypox:interim guidance, 24 June 2022. https://apps.who.int/iris/handle/10665/357186.

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      Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus

        Corresponding author: Tao Jiang, jiangtao@bmi.ac.cn
        Corresponding author: Xiaoping Kang, kangxiaoping@bmi.ac.cn
      • a. State Key Laboratory of Pathogen and Biosecurity, The Academy of Military Medical Science, Institute of Microbiology and Epidemiology, Beijing, 100071, China;
      • b. Laboratory Department of the First Medical Center, Chinese PLA General Hospital, Beijing, 100850, China
      • Received Date: 26 August 2022
      • Accepted Date: 02 December 2022

      Abstract: Highlights
      1. Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection.
      2. The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA,
      3. No cross-reaction was found in 14 rash and fever-associated viruses.
      4. The two RPA methods developed are simple and rapid, making them potentially useful for detection of MPXV in the field/clinic.

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