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Volume 01 Issue 1
February 1986
    Citation: DING Da-Ming, WU Ya-Ming, FANG Ci-Qi, ZHOU Yong-Zhi, SUN Guo-Ping, ZHANG Yi-Min, JIAN Hao-Ran. IDENTIFICATION AND MOLECULAR CLONING OF DNA OF BUZURA SUPPRESSARIA NUCLEAR POLYHEDROSIS VIRUS .VIROLOGICA SINICA, 1986, 1(1) : 31.
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    IDENTIFICATION AND MOLECULAR CLONING OF DNA OF BUZURA SUPPRESSARIA NUCLEAR POLYHEDROSIS VIRUS

    • 1.

      Wuhan Institute of Virology

    • 2.

      Academia Sinica

    • Available online: 05 January 1986
    • The DNA of Buzura Suppressaria Nuclear Polyhedrosis Virus(BsNPV) was digested at least9 fragments by restriction endonuclease BamHI.The fragments eluted by polyacrylamide gel electrophoresis were recombined with plasmid pBR_(322)incubated with BamHI and transfered into E.coli. Antibiotic screening,electrophoresis analysis and colony hybridization showed the existence of insert sequence in hybrid plasmid.The result suggested that the cloning of BamHI fragments of BsNPV DNA be successful.
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      IDENTIFICATION AND MOLECULAR CLONING OF DNA OF BUZURA SUPPRESSARIA NUCLEAR POLYHEDROSIS VIRUS

      • 1. Wuhan Institute of Virology
      • 2. Academia Sinica

      Abstract: The DNA of Buzura Suppressaria Nuclear Polyhedrosis Virus(BsNPV) was digested at least9 fragments by restriction endonuclease BamHI.The fragments eluted by polyacrylamide gel electrophoresis were recombined with plasmid pBR_(322)incubated with BamHI and transfered into E.coli. Antibiotic screening,electrophoresis analysis and colony hybridization showed the existence of insert sequence in hybrid plasmid.The result suggested that the cloning of BamHI fragments of BsNPV DNA be successful.

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