PROPAGATION OF EHF VIRUS IN CELL CULTURES OF MERIONES UNGUICULATUS LUNG AND KIDNEY TISSUES
Abstract: 3 strains of epidemic hemorrhagic fever(EHF)virus from lung tissues of Apodemus agrarius and Rattus norvegicus and blood of patient with EHF were inoculated in primary cell culture of Meriones unguiculatus lung and kidney tissues.In the first passage,a good multiplicaton was observed and TCID 50/ml of the primary cell culture of kidney tissues were 10~(6?0) (for R_3 strain)and 10~(6?5)(for B_5 strain).After passage 3,on the 2nd day after inoculation,specific immunofluorescence to EHF virus antigen was detec...