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Volume 01 Issue 2
April 1986
    Citation: ZHANG Cheng-Liang, ZHANG Zuo-Fang, JUE Xiao-Feng, CHEN Jing, HU Wei-Zhen, SONG Shu-Min, LIU Jia-Jue, SUN Yue-Ying, CHEN Bo-Quan, TIAN Bo, JI Liang. ANALYSIS BY MONOCLONAL ANTIBODY OF TOBACCO MOSIAC VIRUS DETERMINANT AND ITS ANTIGEN TYPE .VIROLOGICA SINICA, 1986, 1(2) : 32.
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    ANALYSIS BY MONOCLONAL ANTIBODY OF TOBACCO MOSIAC VIRUS DETERMINANT AND ITS ANTIGEN TYPE

    • 1.

      1.Institute of plant Quarantine

    • 2.

      Ministry of Agriculture

    • 3.

      Animal Husbandry and Fishery

    • 4.

      Institute of Microbiology Academia Sinica.

    • Available online: 05 June 1986
    • Fourteen isolates of Tobacco Mosiac Virus(TMV)were collected from 9 different regions of China.They were multiplied under isolating con- ditions and purified and measured by the same method.The concentra- tion series were started from 20μg/ml and then were diluted into nine dilutions from 10~(-1).10~(-2)…to 10~(-9)(contrasted with healthy host).Each was reacted individually with 10 TMV monoclonal antibodies of optimal dilution(selected through detecting titers)in ELISA(contrasted with poly- clonal antibody ...
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      ANALYSIS BY MONOCLONAL ANTIBODY OF TOBACCO MOSIAC VIRUS DETERMINANT AND ITS ANTIGEN TYPE

      • 1. 1.Institute of plant Quarantine
      • 2. Ministry of Agriculture
      • 3. Animal Husbandry and Fishery
      • 4. Institute of Microbiology Academia Sinica.

      Abstract: Fourteen isolates of Tobacco Mosiac Virus(TMV)were collected from 9 different regions of China.They were multiplied under isolating con- ditions and purified and measured by the same method.The concentra- tion series were started from 20μg/ml and then were diluted into nine dilutions from 10~(-1).10~(-2)…to 10~(-9)(contrasted with healthy host).Each was reacted individually with 10 TMV monoclonal antibodies of optimal dilution(selected through detecting titers)in ELISA(contrasted with poly- clonal antibody ...

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