Citation: LI Cheng-Ming, CHEN Li-Li, YIN Da-Chang, CHEN Dan-Lin, LIU Li-Hua, WANG Hong-Xi, HU Ping. ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN VITRO .VIROLOGICA SINICA, 1986, 1(4) : 30.

ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN VITRO

  • Available online: 05 December 1986
  • A Strain of hepatitis A virus(S_(84-1))was directly isolated from a fecal specimen of acute patient with hepatitis A and serially passaged in human diploid fibroblast(2BS). Some conditions of cultivation of this virus were also studied. Following serial propagation, the incubation time of S_(84-1) virus was reduced from one and half months(passage 2) to 3 days(passage 12). S_(84-1) virus, without cytopathie effect, can t be detected in supernatant fluid by ELISA and was harvested by freezing-throwing the ce...

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    ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN VITRO

    • 1. Department of Viral Disease of Control
    • 2. Health and Anti-epldemic Station of Sichuan Province

    Abstract: A Strain of hepatitis A virus(S_(84-1))was directly isolated from a fecal specimen of acute patient with hepatitis A and serially passaged in human diploid fibroblast(2BS). Some conditions of cultivation of this virus were also studied. Following serial propagation, the incubation time of S_(84-1) virus was reduced from one and half months(passage 2) to 3 days(passage 12). S_(84-1) virus, without cytopathie effect, can t be detected in supernatant fluid by ELISA and was harvested by freezing-throwing the ce...

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