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Volume 02 Issue 1
February 1987
    Citation: LIU Le-He, LI Shao-Bing, MAI Xiao-Ping, GUO Hui-Yu, CAI Shang-Da. PRODUCTION OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODIES TO DENGUE VIRUS TYPE Ⅰ .VIROLOGICA SINICA, 1987, 2(1) : 20.
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    PRODUCTION OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODIES TO DENGUE VIRUS TYPE Ⅰ

    • 1.

      Zhongshan Medical University

    • 2.

      Guangzhou

    • Available online: 05 March 1987
    • Mouse lymphocyte hybridomas were prepared by polyethylene glycolmediated fusion of ceils from a mouse plasmaeytoma line (X63-Ag8-653) with lymphoeytes from Balb/c mice hyperimmunized with dengue virus type Ⅰ (Hawaii). Seven mouse lymphocyte hybridoma clones(C4, F5, F1, A2, B4, H7,D6) which can steadily produce antibodies against dengue-1 antigen were established after selecting and cloning for at least 3 times and have been kept in the laboratory for more than 5 months. Cross-immunofluorescent staining test...
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      PRODUCTION OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODIES TO DENGUE VIRUS TYPE Ⅰ

      • 1. Zhongshan Medical University
      • 2. Guangzhou

      Abstract: Mouse lymphocyte hybridomas were prepared by polyethylene glycolmediated fusion of ceils from a mouse plasmaeytoma line (X63-Ag8-653) with lymphoeytes from Balb/c mice hyperimmunized with dengue virus type Ⅰ (Hawaii). Seven mouse lymphocyte hybridoma clones(C4, F5, F1, A2, B4, H7,D6) which can steadily produce antibodies against dengue-1 antigen were established after selecting and cloning for at least 3 times and have been kept in the laboratory for more than 5 months. Cross-immunofluorescent staining test...

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