Citation: ZHANG Hua-Yuan, RICHARD J.DAEMER. DETECTION OF HEPATITIS A ANTIGEN BY A CELL CULTURE FLUORESCENT ANTIBODY TECHNIQUE WITH BIOTIN-AVIDIN .VIROLOGICA SINICA, 1987, 2(3) : 13.

DETECTION OF HEPATITIS A ANTIGEN BY A CELL CULTURE FLUORESCENT ANTIBODY TECHNIQUE WITH BIOTIN-AVIDIN

  • Available online: 05 September 1987
  • This paper describes an improved fluorescent antibody technique for the detection of HAV. The assay is unique in its use of the characteristic of a highaffinity complex between fluorescence-conjugated avidin and biotin-linked anti-HAV to increase sensitivity. We have made a comparison between direct-fluorescent and fluorescentantibody technique with biotin-avidin with same lot IgG antibody and same lot ACMK inoculated HAV. Because of the high binding affinity of this system due to multiple binding of biotin...

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    DETECTION OF HEPATITIS A ANTIGEN BY A CELL CULTURE FLUORESCENT ANTIBODY TECHNIQUE WITH BIOTIN-AVIDIN

    • 1. National Institute for the Control of Pharmaceutical and Biological Products
    • 2. BeijingNational Center for Drugs and Biologics

    Abstract: This paper describes an improved fluorescent antibody technique for the detection of HAV. The assay is unique in its use of the characteristic of a highaffinity complex between fluorescence-conjugated avidin and biotin-linked anti-HAV to increase sensitivity. We have made a comparison between direct-fluorescent and fluorescentantibody technique with biotin-avidin with same lot IgG antibody and same lot ACMK inoculated HAV. Because of the high binding affinity of this system due to multiple binding of biotin...

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