STUDY OF VARIABLES AFFECTING RUBELLA VIRUS HEMAGGLUTINATION AND HEMAGGLUTINATION INHIBITION TEST

The variables which affect the qualitative and quantitative aspects of rubella virus hemagglutination were defined. Large amount of high titered hemagglutinin was obtained by replacing the supernatant of rubella virus infected BHK_21 cells with fresh medium daily. The optimal result wasobtaitied by using Auletta s buffer, pH6.2. Kaolin and Heparin-MnCl_2 were employed to the treatment of serum samples in hemagglutination inhibition test. Among the 107 serum samples tested, the agreement was 98％. Kaolin trea...