Two-Dimensional Electrophoretic Analysis of Structural Polypeptides of the Bombyx mori Nuclear Folyhedrosis Virus
Abstract: The structural polypeptides of the singly-enveloped viral particles and nuclcocapsids of the Bombyx mori nuclear polyhedrosis virus were analyzed by O Farrell s two-dimensional electrophoresis technique and silver staining. The singly-enveloped viral particle contains ca. 96 polypeptides and the nucleocapsid contains ca. 72 polypeptides. There is a significant amount of a polypeptide with mol. wt. of 31K, beth in the viral particle preparation and in the nucleocapsid preparation, which could not be removed ...