Assay for HSV-Induced Deoxythymidine Kinase and Its Application to Antiviral Compound Study
Abstract: An assay for herpes simplex virus type 1 and 2 induced deoxythymidine (dTK) was described by using ~(125)Ⅰ-UdR as substrate. The assay was also used for detecting the sensitivity of HSV-1 and HSV-2 induced dTK to DHPG. The result showed that sensitivity of viral dTK to DHPG was higher than HeLa cell-dTK s. In addition, we thought that toxicity of DHPG to host cells might be related to the low sensitivity of cell-dTK to DHPG.