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Citation: XIAO Cheng-Zu, ZHANG Ling-An, KONG Wei-Wei, WANG Hong-Xia. Proliferation of Cells and Virus with Microcarrier Suspension Culture System [J].VIROLOGICA SINICA, 1988, 3(2) : 160.

Proliferation of Cells and Virus with Microcarrier Suspension Culture System

  • We have successfully cultured three cell lines BHK-13, BHK-21 and CHO-KI with MC-1 type microcarrier made in our Academy. When the microcarrier was in concentration of 5mg/ml, the cell density was about 30×10~4 cells/ml, all of them could proliferate to 1×10~6 cells/ml after 3-day suspension culture. At this time, inoculated VSV diluted 10 or 10~2 times, incubated at 37℃ for 20-24hs, the viral titer in these media were about 6 Log TCID_(50) or much higher. The yield of CHO-KI cell was the highest. Its titre...

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    Proliferation of Cells and Virus with Microcarrier Suspension Culture System

    • 1. Institute of Microbiology and Epidemiology
    • 2. Academy of Military Medical Sciences

    Abstract: We have successfully cultured three cell lines BHK-13, BHK-21 and CHO-KI with MC-1 type microcarrier made in our Academy. When the microcarrier was in concentration of 5mg/ml, the cell density was about 30×10~4 cells/ml, all of them could proliferate to 1×10~6 cells/ml after 3-day suspension culture. At this time, inoculated VSV diluted 10 or 10~2 times, incubated at 37℃ for 20-24hs, the viral titer in these media were about 6 Log TCID_(50) or much higher. The yield of CHO-KI cell was the highest. Its titre...

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