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Volume 03 Issue 3
June 1988
    Citation: LI Cheng-Meng, YAN Da-Chang, WANG Hong-Xi, LIU Li-Hua, CHEN Dan-Lin, ZENG Xi-Hua, BI Xiao, CHEN Li-Li. Studies on Monoclonal Antibodies to Hepatitis A Virus .VIROLOGICA SINICA, 1988, 3(3) : 272.
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    Studies on Monoclonal Antibodies to Hepatitis A Virus

    • 1.

      Departmynt of Viral Disease Control

    • 2.

      Health and Antiepidemic Centre of Sichuan Province

    • Available online: 05 September 1988
    • Three strain hybridomas (AG3, AD2, AE8) were developed by cell fusion technique. They secreted stably MeAbs against HAV with the titer of 1: 128000—1: 1024000 in ascites fluid by ELISA. All of these McAbs are of positive reaction on HAV derived from patient s stools and cell culture, and this reaction can be inhibited by human anti HAV positive sera. The results of cell neutralization test and cross-blocking ELISA show that AE8 McAb is able to neutralize HAV infectivity, and that these McAbs recognize diffe...
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      Studies on Monoclonal Antibodies to Hepatitis A Virus

      • 1. Departmynt of Viral Disease Control
      • 2. Health and Antiepidemic Centre of Sichuan Province

      Abstract: Three strain hybridomas (AG3, AD2, AE8) were developed by cell fusion technique. They secreted stably MeAbs against HAV with the titer of 1: 128000—1: 1024000 in ascites fluid by ELISA. All of these McAbs are of positive reaction on HAV derived from patient s stools and cell culture, and this reaction can be inhibited by human anti HAV positive sera. The results of cell neutralization test and cross-blocking ELISA show that AE8 McAb is able to neutralize HAV infectivity, and that these McAbs recognize diffe...

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