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Citation: CHEN Wei-Mei, LIN Xi-Feng, JIN Hong, LU Wen-Jun. Studies on the Homology of Attacus ricini Nuclear Polyhedrosis Virus and Polyhedrin Gene of Autographa californica Nuclear Polyhedrosis Virus .VIROLOGICA SINICA, 1988, 3(3) : 293.

Studies on the Homology of Attacus ricini Nuclear Polyhedrosis Virus and Polyhedrin Gene of Autographa californica Nuclear Polyhedrosis Virus

  • Available online: 05 September 1988
  • The recombinant DNA (PMA-VI DNA) containing cDNA of mRNA of polyhedringene of Autographa californica nuclear polyhedrosis virus(AcNPV) was used to transform E.coli RR_1, and colonies were selected by antibiotic resistance and colony hybridization.The colony containing PMA-VI plasmid grew in L.Broth.The plasmid DNA was rapidly isolated by boiling method, and tben recovered by electroelution. With the plasmid DNA as a probe and by Southern hybridization, the results showed that the sequence homologues to the ...

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    Studies on the Homology of Attacus ricini Nuclear Polyhedrosis Virus and Polyhedrin Gene of Autographa californica Nuclear Polyhedrosis Virus

    • 1. Dept.of Virology.Wvhan University
    • 2. Wuhan

    Abstract: The recombinant DNA (PMA-VI DNA) containing cDNA of mRNA of polyhedringene of Autographa californica nuclear polyhedrosis virus(AcNPV) was used to transform E.coli RR_1, and colonies were selected by antibiotic resistance and colony hybridization.The colony containing PMA-VI plasmid grew in L.Broth.The plasmid DNA was rapidly isolated by boiling method, and tben recovered by electroelution. With the plasmid DNA as a probe and by Southern hybridization, the results showed that the sequence homologues to the ...

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