Citation: MA Xiu-Li, XIONG Xue-De, XIONG Ke-Yong, TAN Hui-Qing, ZHOU Yong-Zhi. An Effecive procedure for the Separation and Preparation of Citrus Exocortis Viroid .VIROLOGICA SINICA, 1988, 3(4) : 370.

An Effecive procedure for the Separation and Preparation of Citrus Exocortis Viroid

  • Available online: 05 December 1988
  • Viroid is small and exists less in host, which is difficult to purify. General method of purification is phenol extract, remove carbohydrate, dialysis, LiCl partition, DNase degradation and cellulose chromatography. The procedure is complicated, recovery ratio is less and the pure sample which is adapted to all kinds of use can t be gotten. A procedure for the separation and preparation of citrus exocortis viroid (CEV)is reported Nucleic acids were extracted from citrus leaves by phenolchloroform treament T...

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    An Effecive procedure for the Separation and Preparation of Citrus Exocortis Viroid

    • 1. Wuhan Institute of Virology
    • 2. Academia Sinica

    Abstract: Viroid is small and exists less in host, which is difficult to purify. General method of purification is phenol extract, remove carbohydrate, dialysis, LiCl partition, DNase degradation and cellulose chromatography. The procedure is complicated, recovery ratio is less and the pure sample which is adapted to all kinds of use can t be gotten. A procedure for the separation and preparation of citrus exocortis viroid (CEV)is reported Nucleic acids were extracted from citrus leaves by phenolchloroform treament T...

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