Preparation of α-~(32)P HBV DNA Probe and its Application in the Serum Detection

Hepatitis B virus(HBV) DNA purified from the cloned plasmid pHBV_1 and labelled by nick-translation with α-~(32)p dCTP was as probe, which can be hybridized specifically with HBV DNA. The minimum amount about 0.2pg of DNA has been detected by high specific activity probes. The range of the isotope incorporation rates were 23.5-67.0 per cent. The probes s specific activities were 1-1.50×10~8cpm/μg DNA. According to clinical statistics the positive proportions of HBV DNA Correspond to 53.3% 69.7% 78.6% and 81...