Method for Preparation of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Antigens by Treatment Infected Mouse Brains and Lungs with Tween-ether

We first utilized infected mouse brains and lungs to prepare HFRSV specific antigens by treatment with Tween 80-ether. This procedure was very economical and easy. The T-E antigens was a more sensitive, uniform reagent for the RPHI or ELISA and non-multiplication. It also showed an increased thermal stablity at different temperatures and could be stored for more than one year at and below 4℃ without loss of antigenic activity.