Experimental Research and Application of Polyacrylamide Gel Debris Absorption and Elution Method for Virus Concentration
Abstract: This paper reports the results of experimental research and application of polyacrylamide gel(PAG) debris absorption and elution method for Newcastle Disease Virus (NDV) and Human Rotavirus(HRV) concentration. By treating once and twice with PAG, the volume of diluted NDV infected allantoic fluids was concentrated about 12.1 and 90.2 folds respectively, and the HA titer of the virus was increased correspondingly. It was shown that the positive dilution titer of RNA electrophoretype detected in HRV faecal ex...