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Volume 04 Issue 4
August 1989
    Citation: CHEN Huo-Sheng, GUO Hui-Yu, LUO Jin-Xian, HU Jin-Xin. Detection of Dengue Virus RNA by DNA-RNA Dot-spot Hybridization .VIROLOGICA SINICA, 1989, 4(4) : 365.
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    Detection of Dengue Virus RNA by DNA-RNA Dot-spot Hybridization

    • 1.

      Department of Microbiology and Immunology

    • 2.

      Sun Yat-sen University of Medical Sciences

    • 3.

      GuangzhouBiotechnology Research Center

    • Available online: 05 December 1989
    • Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA of dengue virus 2 RNA. Using the [a-~(32)p] dCTP labelled pEH 920 DNA as the probe, dengue virus RNA in the supernatants of infected C6/36 mosquito cells have been detected by DNA-RNA dot-spot hybridization. The results show that the probe strongly reacted with dengue 2 virus RNA though it also cross-hybridized with dengue 1, 3 and 4 virus RNA. The preliminary experiments show that the probe can at least de...
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      Detection of Dengue Virus RNA by DNA-RNA Dot-spot Hybridization

      • 1. Department of Microbiology and Immunology
      • 2. Sun Yat-sen University of Medical Sciences
      • 3. GuangzhouBiotechnology Research Center

      Abstract: Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA of dengue virus 2 RNA. Using the [a-~(32)p] dCTP labelled pEH 920 DNA as the probe, dengue virus RNA in the supernatants of infected C6/36 mosquito cells have been detected by DNA-RNA dot-spot hybridization. The results show that the probe strongly reacted with dengue 2 virus RNA though it also cross-hybridized with dengue 1, 3 and 4 virus RNA. The preliminary experiments show that the probe can at least de...

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