Isolation of Enterovirus Type 71 from the Vesicle Fluid of an Adult Patient with Hand-footmouth Disease in China
Abstract: Isolation and characterization of a H isolate of enterovirus type 71 (E71) from the vesicle fluid of hand-foot-mouth disease have been reported in present study.The H isolate can grow at primary human embryo lung cells, MA104 cells, and BSC cells and results in a typical enterovirus cytopathic effect. 1-day-old mice inoculated ip with H isolate showed limb paralysis and died at 6th day postinfection. Numerous mature virus particles with a diameter of 25 nm in crystal arrays in the cytoplasm have been visualized under microscope. The paired sera of the patient have been tested and showed a 4-fold increase of neutralizing antibody to H isolate. Using 100 antibody units of anti-Cox A5, 7, 9, 16, E70, E71, and 50 antibody units of enterovirus LBM typing pools A-H as well as McAb p27 of E71 BrCr strain, H isolate could be only neutralized by both anti-E71 polyclone and monoclone antibodies. The minimal effective antibody units to H isolate are required respectively 1.6 for anti-E71 polyclone antibody and 64 for McAb p27. Furthermore, high titers of anti-Cox A16 have also showed some cross reaction to H isolate shares a common antigen with Cox A16.