A Plaque Assay under Normal Atmosphere

A method for plaquing Grass Carp Hemorrhage Virus-873 has been studied. We tested inorganic(NaHCO_3) and organic(Tris, HEPES) buffer systems in open dish culture and found the combination of organic buffers with bicarbonate to be very promising for both cell growth and viral replication The CIK cells growed well in three media (MEM-NaHCO_3, MEM-Tris and MEMHEPES)under normal atmosphere or 5% CO_2 presure, but the organic buffer alone provided a little good cell growth respose. Two-phase (gel-liquid)media in...