Citation: MENG Ji-Hong, ZHU Sheng-He, SUN Yong-Liang. Studies on Semimicro Quick Monolayer Plaque Assay of Viruses .VIROLOGICA SINICA, 1990, 5(4) : 341.

Studies on Semimicro Quick Monolayer Plaque Assay of Viruses

  • Available online: 05 December 1990
  • A semimicro quick monolayer plaque assay of viruses (SQMPAV) without preparing the monolayer in advance was developed. In this assay, the cells and viruses were simultaneously inoculated into the wells of multi-well tissue culture plates. So the attachment of cells and the adsorption of viruses occurred at the same time. Then, the plaques were produced as the monolayers were formed under the nutrient gel overlay. With the model of polioviruses, a series of studies on SQMPAV, including the experimental condi...

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    Studies on Semimicro Quick Monolayer Plaque Assay of Viruses

    • 1. Department of Microbiology
    • 2. Nanjing Railway Medical College
    • 3. Nanjing

    Abstract: A semimicro quick monolayer plaque assay of viruses (SQMPAV) without preparing the monolayer in advance was developed. In this assay, the cells and viruses were simultaneously inoculated into the wells of multi-well tissue culture plates. So the attachment of cells and the adsorption of viruses occurred at the same time. Then, the plaques were produced as the monolayers were formed under the nutrient gel overlay. With the model of polioviruses, a series of studies on SQMPAV, including the experimental condi...

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