Detection of Specific IgM from Patients with Epidemic Parotitis by Enzyme Linked Immunosorbent Assay
Abstract: Specific IgM from patients with epidemic parotitis ( EP ) was detected by enzyme linked immunosorbent assay (ELISA). The mumps virus antigen was prepared by using mumps virus Ender s strain. The detected IgM antibody was confirmed by 2-EM destruction. Sensitibity of the assay for mumps virus-IgM was high as showing a titer of 1:12800 in the positive sera from the patients. In clinically diagnosed 79 cases from the patients with epidemic parotitis, 72 cases were IgM positive, and the positive rate was 91%. I...