Molecular Cloning of Bovine Herpesvirus Type 4 (DN-599 Strain) DNA
Abstract: Bovine herpesvirus type 4 (BHV-4) DNA was extracted from infected MDBK (Bovine kidney) cells using Triton Ⅹ-100 and phenol/chloroform/ether. With shotgun and selective cloning procedures, the viral DNA was cloned into EcoR I, BamH I or Hind III sites of plasmids pBR322 or pUC9 after digestion of the DNA with the three restriction endonucleases, respectively, and three DNA libraries containing different restricted fragments were constructed. Photobiotin-labeled viral DNA and bovine thymus DNA were used as pr...