Citation: TONG Guang-Zhi, Dieter Burger, David T Shen. Molecular Cloning of Bovine Herpesvirus Type 4 (DN-599 Strain) DNA .VIROLOGICA SINICA, 1990, 5(4) : 423.

Molecular Cloning of Bovine Herpesvirus Type 4 (DN-599 Strain) DNA

  • Available online: 05 December 1990
  • Bovine herpesvirus type 4 (BHV-4) DNA was extracted from infected MDBK (Bovine kidney) cells using Triton Ⅹ-100 and phenol/chloroform/ether. With shotgun and selective cloning procedures, the viral DNA was cloned into EcoR I, BamH I or Hind III sites of plasmids pBR322 or pUC9 after digestion of the DNA with the three restriction endonucleases, respectively, and three DNA libraries containing different restricted fragments were constructed. Photobiotin-labeled viral DNA and bovine thymus DNA were used as pr...

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    Molecular Cloning of Bovine Herpesvirus Type 4 (DN-599 Strain) DNA

    • 1. Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences
    • 2. Harbin 150001USDA-ARS
    • 3. Washington State University

    Abstract: Bovine herpesvirus type 4 (BHV-4) DNA was extracted from infected MDBK (Bovine kidney) cells using Triton Ⅹ-100 and phenol/chloroform/ether. With shotgun and selective cloning procedures, the viral DNA was cloned into EcoR I, BamH I or Hind III sites of plasmids pBR322 or pUC9 after digestion of the DNA with the three restriction endonucleases, respectively, and three DNA libraries containing different restricted fragments were constructed. Photobiotin-labeled viral DNA and bovine thymus DNA were used as pr...

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