Citation: TUN Zhong-Xin, CAI Yi-Quan. Identification of Baculovirus by Dot Hybridization .VIROLOGICA SINICA, 1991, 6(1) : 49.

Identification of Baculovirus by Dot Hybridization

  • Available online: 05 March 1991
  • The polyhedrin gene of Prodenia lituea nuclear polyhedrosis virus(PlNPV) was mapped on the PlNPV DNA HindⅢ-A fragment by Southern-blot hybridization with the AcNPV DNA EeoRI-Ⅰ fragment as probe under 35℃ hybridization conditions. Then, two probes were prepared from PINPV DNA HindⅢ-A and PINPV DNA HindⅢ-C fragments respectively for the identification of PINPV DNA, MbNPV DNA, BsNPV DNA under different hybridization conditions. The results showed that the probe containing the polyhedrin gene was more extensive...

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    Identification of Baculovirus by Dot Hybridization

    • 1. Wuhan Institute of Virology
    • 2. Academia Sinica

    Abstract: The polyhedrin gene of Prodenia lituea nuclear polyhedrosis virus(PlNPV) was mapped on the PlNPV DNA HindⅢ-A fragment by Southern-blot hybridization with the AcNPV DNA EeoRI-Ⅰ fragment as probe under 35℃ hybridization conditions. Then, two probes were prepared from PINPV DNA HindⅢ-A and PINPV DNA HindⅢ-C fragments respectively for the identification of PINPV DNA, MbNPV DNA, BsNPV DNA under different hybridization conditions. The results showed that the probe containing the polyhedrin gene was more extensive...

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