Rapid Detection of Bean Yellow Mosaic Virus, Potato Virus M and Oat Mosaic Virus Using Immunosorbent Electronmicroscopy

Bean yellow mosaic virus (BYMV), potato virus M(PVM) and oat mosaic virus (OMV)were rapidly detected in infected leaf extracts using immunosorbent electron microscopy. The suitable dilution and time for coating the three antisera in carbon coated grids were 1000 and 1h at room temperature. The suitable trapping time for BYMV, PVM and OMV were 2,2 and 8h respectively at 4℃. The detective sensitivities in leaf saps were 4000 dilutions for PVM and OMV, and a few virus particles could still be seen at the dilut...