Citation: LIU Ming-Fu, XIE Tian-En. Comparative Studies on Two Similar Proteins of Buzura suppressaria Nuclear Polyhedrosis Virus with Chemical Cleavage Method in situ .VIROLOGICA SINICA, 1991, 6(1) : 75.

Comparative Studies on Two Similar Proteins of Buzura suppressaria Nuclear Polyhedrosis Virus with Chemical Cleavage Method in situ

  • Available online: 05 March 1991
  • Aspartyl-Prolyl bonds in proteins could be selectively hydrolyzed with 75% formic aeid. The in situ aeidie cleavage procedure based on acid lability of Asp-Pro bonds is very important in sequence analysis of proteins and comparative studies of related or similar proteins, Limited cleavage of polyhedrin and virion 31k polypeptide which have the same molecalar weights in SDS-PAGE of Buzura suppresaria nuclear polyhedrosis virus by above method produced 4 and 8 bands, respectively. The results suggest that the...

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    Comparative Studies on Two Similar Proteins of Buzura suppressaria Nuclear Polyhedrosis Virus with Chemical Cleavage Method in situ

    • 1. Wuhan Institute of Virology
    • 2. Academia Sinica

    Abstract: Aspartyl-Prolyl bonds in proteins could be selectively hydrolyzed with 75% formic aeid. The in situ aeidie cleavage procedure based on acid lability of Asp-Pro bonds is very important in sequence analysis of proteins and comparative studies of related or similar proteins, Limited cleavage of polyhedrin and virion 31k polypeptide which have the same molecalar weights in SDS-PAGE of Buzura suppresaria nuclear polyhedrosis virus by above method produced 4 and 8 bands, respectively. The results suggest that the...

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