Citation: ZHAO Hong-Li, HONG Mei-Xian, JIANG Chao-Zhun, LIU Dun-Bin, AN Jia-Ze, LV Hai, LIU Jun-Lian. Experimental Diagnosis of HFRS with Photobiotin-Labelled cDNA Probe .VIROLOGICA SINICA, 1991, 6(3) : 214.

Experimental Diagnosis of HFRS with Photobiotin-Labelled cDNA Probe

  • Available online: 05 September 1991
  • Hemorrhagic fever with renal syndrome(HFRS)viral RNA in specimens of urine cells, serum and peripherial blood lymphoeytes(PBL)from patients was detected by dot-blot hybridization with photobiotin-labelled Rs cDNA probe. Positive results were obtained with 52 of 81 urine, 24 of 48 serum and 30 of 42 PBL specimens. Hybridization signal was not found in the specimens from those without HFRS of 55 urine,30 sera and 15 PBL. The positive rate of the three kinds specimens were not significantly different (P>0.0...

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    Experimental Diagnosis of HFRS with Photobiotin-Labelled cDNA Probe

    • 1. Department of Microbiology
    • 2. The Fourth Military Medical University

    Abstract: Hemorrhagic fever with renal syndrome(HFRS)viral RNA in specimens of urine cells, serum and peripherial blood lymphoeytes(PBL)from patients was detected by dot-blot hybridization with photobiotin-labelled Rs cDNA probe. Positive results were obtained with 52 of 81 urine, 24 of 48 serum and 30 of 42 PBL specimens. Hybridization signal was not found in the specimens from those without HFRS of 55 urine,30 sera and 15 PBL. The positive rate of the three kinds specimens were not significantly different (P>0.0...

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