Citation: YANG Tie-Qiu, LI Zhi-Hong, YANG Beng, LIU Wei-Qian, DIAO Wen-Xian, LI De-Xin, HANG Chang-Shou. Detection of Epidemic Hemorrhagic Fever Virus (Hantanvirus) RNA Genome with Biotin-Labeled Probe by Dot Hybridization .VIROLOGICA SINICA, 1991, 6(4) : 320.

Detection of Epidemic Hemorrhagic Fever Virus (Hantanvirus) RNA Genome with Biotin-Labeled Probe by Dot Hybridization

  • Available online: 05 December 1991
  • Dot hybridization assay was developed to detect epidemic hemorrhagic fever virus(EHFV)RNA genome using probe which biotin-7-dATP labeled R-3 cDNA clone of M fragement of EHFV R-22 strain. This probe was used to detect RNA genome of the human original EHFV H-114 and H-435 strains in cell cultures. The positive hybridization signal was obtained from cells infected EHFV H-114 and H-435 strains, 5 pg cDNA or RNA could be obtained This probe do not react with DNA of cells infected herpes simplex virus and cytome...

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    Detection of Epidemic Hemorrhagic Fever Virus (Hantanvirus) RNA Genome with Biotin-Labeled Probe by Dot Hybridization

    • 1. Virus Research Institute
    • 2. Hubei Medical College
    • 3. Wuhan

    Abstract: Dot hybridization assay was developed to detect epidemic hemorrhagic fever virus(EHFV)RNA genome using probe which biotin-7-dATP labeled R-3 cDNA clone of M fragement of EHFV R-22 strain. This probe was used to detect RNA genome of the human original EHFV H-114 and H-435 strains in cell cultures. The positive hybridization signal was obtained from cells infected EHFV H-114 and H-435 strains, 5 pg cDNA or RNA could be obtained This probe do not react with DNA of cells infected herpes simplex virus and cytome...

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