Detection of HIV-1 DNA by PCR

A single pair of oligonucleotide primers within the envelope gene of HIV1(primer 1: 5 -AGCAGCAGGAAGCACTATGGGC-3 ; primer 2: 5 -CCAGACTGAGTTGCAACA-3 ), 142bp apart, were synthesized and used to amplify a specific fragment of the HIV-1 DNA in infected cell cultures(MT4 HIV 1), and in plasmid pⅢexE7 carrying HIV-1 envelope gene by means of polymerase chain reaction(PCR). Amplified product was identified as 142bp fragment by agrose electrophoresis and cut into a 50bp and a 92bp fragment with restriction endonuc...