Studies on the Antisera to Narcissus Viruses
Abstract: Narcissus mosaic virus(NMV) was obtained from the mixed infected narcissus plants and purified from the inoculated leaves of Chenopodium amarnticolor. The procedures of purification involved precipitation with 6% PEG ( M. W. 6000 ), differential centrifugation and 20—50% sucrose density-gradient centrifugation. After 6 times of immune injection to rabbit with the purified preparation of NMV, an antiserum was prepared with a titre of 1:5000 tested by precipitation reactions in capillary pipettes about 4 week...