Use of Polymerase Chain Reaction for Detection of the Seguence Related to HPV16 E6/E7 in Cervical Cancers
Abstract: Detection of the sequence related to E6/E7 of human papillomavirus type16 in Chinese cervical cancers using polymerase chain reaction (PCR) has beenreported in this paper. We have used the two-step protocol PCR to detectDNA sequence related to HPV16 E6/E7 in 34 cervical cancer tissues, by primingwith SP and ASP, the positive percentage of HPV 16 E6/E7 in cervical cancerswas 67.6% (23/34). This result showed that HPV 16 may play an importantrole in development and progression of cervical cancers, and u...