Citation: WU Yun-Tao, KE Li-Hua, CAI Yi-Quan. Identification of PINPV--DNA Recombinant Colonies with Sandwich Hybridization .VIROLOGICA SINICA, 1992, 7(1) : 63.

Identification of PINPV--DNA Recombinant Colonies with Sandwich Hybridization

  • Available online: 05 March 1992
  • The genome of the bacularvirus PINPV was digested completely into sevenfragment (A、B、C、D_1、D_2、E、F) with Hind Ⅲ. These fragments were ligated in vitro with vector pUC18 seperately and used to transform host strainE. coli TG_1. Recombinant colonies were identified with sandwich hybridization.Seven kinds of recombinant colonies containing the PINPV-DNA Hind Ⅲ fra-gments were obtained, but unfortunately the A fragment was less than theoriginal one.

  • 加载中
  • 加载中

Article Metrics

Article views(3968) PDF downloads(860) Cited by()

Related
Proportional views

    Identification of PINPV--DNA Recombinant Colonies with Sandwich Hybridization

    • 1. Wuhan Institute of Virology

    Abstract: The genome of the bacularvirus PINPV was digested completely into sevenfragment (A、B、C、D_1、D_2、E、F) with Hind Ⅲ. These fragments were ligated in vitro with vector pUC18 seperately and used to transform host strainE. coli TG_1. Recombinant colonies were identified with sandwich hybridization.Seven kinds of recombinant colonies containing the PINPV-DNA Hind Ⅲ fra-gments were obtained, but unfortunately the A fragment was less than theoriginal one.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return