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Volume 07 Issue 1
February 1992
    Citation: LIU Ming-Fu, HU Zhi-Hong, JIN Feng, LI Meng-Jin, XIE Tian-En. Localization and Cloning of the Polyhedrin Gene of Buzura suppressaria Nuclear Polyhedrosis Virus .VIROLOGICA SINICA, 1992, 7(1) : 80.
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    Localization and Cloning of the Polyhedrin Gene of Buzura suppressaria Nuclear Polyhedrosis Virus

    • 1.

      Wuhan Institute of Virology

    • 2.

      Academia Sinica

    • Available online: 05 March 1992
    • The polyhedrin gene of tung-oil geometrid, Buzura suppressaria,nuclear polyhedrosis virus(BsNPV) was localized on BamHI-B, BglI-A, Bgl Ⅱ-F, EcoRI-P, HindⅢ-A, KpnI-I, PstI-D, XbaI-A or B and XhoI-F/G fra-gments under low stringence(40% formamide, 35℃), using the recombinantplasmid of AcNPV DNA EcoRI-I as the probe. For sequencing the polyhedringene, the KpnI-I fragment was cloned into M13mp18 vector.
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      Localization and Cloning of the Polyhedrin Gene of Buzura suppressaria Nuclear Polyhedrosis Virus

      • 1. Wuhan Institute of Virology
      • 2. Academia Sinica

      Abstract: The polyhedrin gene of tung-oil geometrid, Buzura suppressaria,nuclear polyhedrosis virus(BsNPV) was localized on BamHI-B, BglI-A, Bgl Ⅱ-F, EcoRI-P, HindⅢ-A, KpnI-I, PstI-D, XbaI-A or B and XhoI-F/G fra-gments under low stringence(40% formamide, 35℃), using the recombinantplasmid of AcNPV DNA EcoRI-I as the probe. For sequencing the polyhedringene, the KpnI-I fragment was cloned into M13mp18 vector.

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