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Volume 07 Issue 2
April 1992
    Citation: TIAN Mu-Zhen, DONG Ji-Hua, TU Fu-Xun, XIE Gong-Bin, CHAI Rui-Zhen. Monoclonal Antibodies to Respiratory Syncytial Virus and Their Application in Identificating the Virus Protein Specificity and Comparing Their Antigenic Difference between Virus Isolates .VIROLOGICA SINICA, 1992, 7(2) : 181.
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    Monoclonal Antibodies to Respiratory Syncytial Virus and Their Application in Identificating the Virus Protein Specificity and Comparing Their Antigenic Difference between Virus Isolates

    • 1.

      Union Hospital

    • 2.

      Tongji Medical University

    • Available online: 05 June 1992
    • The BALB/C mice were immunized with HeLa cells infected with respiratory syncytial virus(RSV, Wuhan strain). The immunized spleen cells were fused with SP2/0 myeloma cells which were obtained from the SP2/0 solid tumor grown in BALB/C mice. Thirteen hybridoma cell lines secreting antiRSV monoclonal antibody(MeAb) were established. The chromosome number of these cell lines was ranged from 94—104 and the seerated MeAbs belonged to routine IgG1, IgG2a, IgG2b subclasses. The ascites antibody titer was from 1: 1...
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      Monoclonal Antibodies to Respiratory Syncytial Virus and Their Application in Identificating the Virus Protein Specificity and Comparing Their Antigenic Difference between Virus Isolates

      • 1. Union Hospital
      • 2. Tongji Medical University

      Abstract: The BALB/C mice were immunized with HeLa cells infected with respiratory syncytial virus(RSV, Wuhan strain). The immunized spleen cells were fused with SP2/0 myeloma cells which were obtained from the SP2/0 solid tumor grown in BALB/C mice. Thirteen hybridoma cell lines secreting antiRSV monoclonal antibody(MeAb) were established. The chromosome number of these cell lines was ranged from 94—104 and the seerated MeAbs belonged to routine IgG1, IgG2a, IgG2b subclasses. The ascites antibody titer was from 1: 1...

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