Serving the Society Since 1986
Advanced Search
Volume 07 Issue 3
June 1992
    Citation: XU Xiu-Ping, ZHAO Wei-Meng, DONG Jie-De, ZHAO Li-Xin, CHEN Chen-Hua, StevenA.Jenison. Clone and Expression of Human Papillomavirus Type 16 L_1 Open Reading Frame .VIROLOGICA SINICA, 1992, 7(3) : 253.
    shu

    Clone and Expression of Human Papillomavirus Type 16 L_1 Open Reading Frame

    • 1.

      Department of Microbiology

    • 2.

      Shandong Medical Univerity

    • Available online: 05 September 1992
    • 人乳头瘤病毒16型(HPV16)含有两个晚期开放读码框(L_1ORF和L_2ORF),L_1ORF编码主要衣壳蛋白,我们用质粒pHPV16、p16L_2BX_5和pATH,采用基因重组技术,制备了含有HPV16个长L_1ORF序列的基因克隆p16L_1BN(5071—253),它能在大肠杆菌中有效表达,产生分子量约90KD的含有E. coli trpE的融合蛋白。Western blot检测,该蛋白可被抗牛乳头瘤病毒的抗体识别,这说明克隆p16L_1BN能有效表达,基因产物具有乳头瘤病毒型的共同抗原的性质。
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(4122) PDF downloads(1001) Cited by()

    Related
    Proportional views

      Clone and Expression of Human Papillomavirus Type 16 L_1 Open Reading Frame

      • 1. Department of Microbiology
      • 2. Shandong Medical Univerity

      Abstract: 人乳头瘤病毒16型(HPV16)含有两个晚期开放读码框(L_1ORF和L_2ORF),L_1ORF编码主要衣壳蛋白,我们用质粒pHPV16、p16L_2BX_5和pATH,采用基因重组技术,制备了含有HPV16个长L_1ORF序列的基因克隆p16L_1BN(5071—253),它能在大肠杆菌中有效表达,产生分子量约90KD的含有E. coli trpE的融合蛋白。Western blot检测,该蛋白可被抗牛乳头瘤病毒的抗体识别,这说明克隆p16L_1BN能有效表达,基因产物具有乳头瘤病毒型的共同抗原的性质。

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return