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Volume 07 Issue 3
June 1992
    Citation: XIE Tian-En, BANG Hui-Yin, CENG Yun-Tian, ZHANG Zhong-Shen, WANG Lu-Meng, ZHANG Yang-Lian, LIU Yu-Lan, JIN Feng, LIU Song-Hua, DENG Wan-Gui, XIAO Zuo-Lv. New Technique for the Production of Viral Insecticide of B. suppressaria Nuclear Polyhedrosis Virus .VIROLOGICA SINICA, 1992, 7(3) : 304.
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    New Technique for the Production of Viral Insecticide of B. suppressaria Nuclear Polyhedrosis Virus

    • 1.

      Wuhan Institute of Virology

    • 2.

      Academia Sinica

    • Available online: 05 September 1992
    • The oil tree moth (B. suppressaria larvae) was grown to 4 instar using a seml-synthetic artificial diet. then infected with (BsNPV) inoculum propagated in the insect cell cultures. 7 days p.i., the virus-infected larvae were collected and virus (PIB) was purified by differential centrifugation. Then, a flowable formulation was made with the glycerin, virus-protective agent, emulsion and antibiotic preparation was determined by standard bioassays using laboratory-reared larvae on artificial diet. LC50 was 2....
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      New Technique for the Production of Viral Insecticide of B. suppressaria Nuclear Polyhedrosis Virus

      • 1. Wuhan Institute of Virology
      • 2. Academia Sinica

      Abstract: The oil tree moth (B. suppressaria larvae) was grown to 4 instar using a seml-synthetic artificial diet. then infected with (BsNPV) inoculum propagated in the insect cell cultures. 7 days p.i., the virus-infected larvae were collected and virus (PIB) was purified by differential centrifugation. Then, a flowable formulation was made with the glycerin, virus-protective agent, emulsion and antibiotic preparation was determined by standard bioassays using laboratory-reared larvae on artificial diet. LC50 was 2....

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