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Volume 07 Issue 3
June 1992
    Citation: BAI Li-Ping, TONG Guang-Zhi, DIAO Yong-Jun, TUN Bao-Cheng, YUE Jun-Meng, WANG Mei, YAN Shen. Restriction Enzyme Analysis and Molecular Cloning of CAV-1 DNA .VIROLOGICA SINICA, 1992, 7(3) : 357.
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    Restriction Enzyme Analysis and Molecular Cloning of CAV-1 DNA

    • 1.

      The National Veterinary biotechnological Laboratory

    • 2.

      Harbin Vet. Res. Inst. Harbin 150001The Military Veterinary Research Institute

    • 3.

      Vet. College of PLA

    • 4.

      Changchun 130012

    • Available online: 05 September 1992
    • The virulent and modified canine adenovirus type 1 (CAV-1) DNAs extracted from canine kidney cells (MDCK) 36 hrs postinfection were both analysed with restriction enzymes EcoR Ⅰ, BamH Ⅰ, Pst Ⅰ, Nsi Ⅰ, Sph Ⅰ and Hind Ⅲ. and no difference was found from the electrophoresis pattern between the two viruses. The modified CAV-1 DNA was digested completely with Pst Ⅰ and cloned into, plasmid pBluescript SK taking shotgun procedure. Through hybridization selection of photobiotin labeled CAV-1 DNA and Pst Ⅰ digestio...
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      Restriction Enzyme Analysis and Molecular Cloning of CAV-1 DNA

      • 1. The National Veterinary biotechnological Laboratory
      • 2. Harbin Vet. Res. Inst. Harbin 150001The Military Veterinary Research Institute
      • 3. Vet. College of PLA
      • 4. Changchun 130012

      Abstract: The virulent and modified canine adenovirus type 1 (CAV-1) DNAs extracted from canine kidney cells (MDCK) 36 hrs postinfection were both analysed with restriction enzymes EcoR Ⅰ, BamH Ⅰ, Pst Ⅰ, Nsi Ⅰ, Sph Ⅰ and Hind Ⅲ. and no difference was found from the electrophoresis pattern between the two viruses. The modified CAV-1 DNA was digested completely with Pst Ⅰ and cloned into, plasmid pBluescript SK taking shotgun procedure. Through hybridization selection of photobiotin labeled CAV-1 DNA and Pst Ⅰ digestio...

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